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This protein carries a mouse IgG1 Fc tag at the C-terminus
The protein has a calculated MW of 64.1 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human Nectin-2, Mouse IgG2a Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human Nectin-2, Mouse IgG2a Fc,Avitag (Cat. No. CD2-H82A3) is more than 85% and the molecular weight of this protein is around 260-275 kDa verified by SEC-MALS.
Immobilized Human PVRIG, His Tag (Cat. No. PVG-H52H4) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Nectin-2, Mouse IgG2a Fc,Avitag (Cat. No. CD2-H82A3) with a linear range of 0.6-10 ng/mL (QC tested).
Immobilized Human DNAM-1, Fc Tag (Cat. No. DN1-H5257) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Nectin-2, Mouse IgG2a Fc,Avitag (Cat. No. CD2-H82A3) with a linear range of 0.01-0.625 μg/mL (Routinely tested).
Immobilized Biotinylated Human Nectin-2, Mouse IgG2a Fc,Avitag (Cat. No. CD2-H82A3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human PVRIG, His Tag (Cat. No. PVG-H52H4) with a linear range of 1-20 ng/mL (Routinely tested).
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