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This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)
The protein has a calculated MW of 24.6 kDa. The protein migrates as 30-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Mouse Fc gamma RIV, His,Avitag (BLI verified) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Biotinylated Mouse Fc gamma RIV, His,Avitag (BLI verified) (Cat. No. FC4-M82E8) was greater than 85% as determined by SEC-HPLC.
Immobilized FMC63 at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse Fc gamma RIV, His,Avitag (BLI verified) (Cat. No. FC4-M82E8) with a linear range of 0.3-20 ng/mL (Routinely tested).
Biotinylated Mouse Fc gamma RIV, His,Avitag (BLI verified) (Cat. No. FC4-M82E8) captured on Biotin CAP - Series S sensor Chip can bind Herceptin with an affinity constant of 0.186 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Biotinylated Mouse Fc gamma RIV, His,Avitag (BLI verified) (Cat. No. FC4-M82E8) captured on Biotin CAP - Series S sensor Chip can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), Ultra-low endotoxin (Cat. No. CDE-M120a) with an affinity constant of 31.7 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Biotinylated Mouse Fc gamma RIV, His,Avitag (BLI verified) (Cat. No. FC4-M82E8) on SA Biosensor, can bind Rituximab with an affinity constant of 0.394 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Price(USD) : $350.00
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