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Your Position: Home > Protein > Fc gamma RI / CD64 > FCA-H82E8

Biotinylated Human Fc gamma RI / CD64 Protein, His,Avitag™ (SPR & BLI & MALS verified)

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  • Synonym
    FCGR1A,FCG1,FCGR1,IGFR1,CD64,CD64A,FCRI
  • Source
    Biotinylated Human CD64, His,Avitag(FCA-H82E8) is expressed from human 293 cells (HEK293). It contains AA Gln 16 - Pro 288 (Accession # P12314-1).
    Predicted N-terminus: Gln 16
  • Molecular Characterization
    Fc gamma RI / CD64 Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 34.2 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
SDS-PAGE
Fc gamma RI / CD64 SDS-PAGE

Biotinylated Human CD64, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Fc gamma RI / CD64 MALS images

The purity of Biotinylated Human CD64, His,Avitag (Cat. No. FCA-H82E8) is more than 90% and the molecular weight of this protein is around 46-66 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Fc gamma RI / CD64 ELISA

Immobilized Ipilimumab at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD64, His,Avitag (Cat. No. FCA-H82E8) with a linear range of 0.1-3 ng/mL (QC tested).

Bioactivity-SPR
 Fc gamma RI / CD64 SPR

Biotinylated Human CD64, His,Avitag (Cat. No. FCA-H82E8) captured on Biotin CAP - Series S sensor Chip can bind Anti-Her2 antibody, Human IgG1 | Human Kappa with an affinity constant of 0.435 nM as determined in a SPR assay (Biacore T200) (QC tested).

 Fc gamma RI / CD64 BATCH SPR
Bioactivity-BLI
 Fc gamma RI / CD64 BLI

Loaded Biotinylated Human CD64, His,Avitag (Cat. No. FCA-H82E8) on SA Biosensor, can bind Herceptin with an affinity constant of 0.515 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

 Fc gamma RI / CD64 BLI

Loaded Biotinylated Human CD64, His,Avitag (Cat. No. FCA-H82E8) on SA Biosensor, can bind Human IgG Fc, His Tag (Cat. No. IG1-H5225) with an affinity constant of 1.60 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Receptors that recognize the Fc portion of IgG are divided into three groups designated Fc gamma RI, RII, and RIII, also known respectively as CD64, CD32, and CD16. Fc gamma RI binds IgG with high affinity and functions during early immune responses. Fc gamma RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses.
    High affinity immunoglobulin gamma Fc receptor I is also known as FCGR1A, FCG1, FCGR1, CD64 and IGFR1, is a type of integral membrane glycoprotein that binds monomeric IgG-type antibodies with high affinity, which belongs to the immunoglobulin superfamily or FCGR1 family. FCGR1A / CD64 contains 3 Ig-like C2-type (immunoglobulin-like) domains. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells.
  • Clinical and Translational Updates

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  • Latest Research Phase:Discontinued

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