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Your Position: Home > Protein > Nectin-2 > CD2-H82F8

Biotinylated Human Nectin-2 / CD112 Protein, Fc,Avitag™ (HPLC verified)

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  • Synonym
    CD112,Nectin-2,PVRL2
  • Source
    Biotinylated Human Nectin-2, Fc,Avitag(CD2-H82F8) is expressed from human 293 cells (HEK293). It contains AA Gln 32 - Leu 360 (Accession # Q92692-2).
    Predicted N-terminus: Gln 32
  • Molecular Characterization
    Nectin-2 Structure

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 64.0 kDa. The protein migrates as 66 kDa under reducing (R) condition, and 130 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Nectin-2 SDS-PAGE

Biotinylated Human Nectin-2, Fc,Avitag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC
Nectin-2 SEC-HPLC

The purity of Biotinylated Human Nectin-2, Fc,Avitag (Cat. No. CD2-H82F8) was greater than 90% as determined by SEC-HPLC.

Bioactivity-ELISA
 Nectin-2 ELISA

Immobilized Human PVRIG, Mouse IgG2a Fc Tag, low endotoxin at 2 μg/mL (100 μL/well) can bind Biotinylated Human Nectin-2, Fc,Avitag (Cat. No. CD2-H82F8) with a linear range of 0.2-13 ng/mL (QC tested).

 Nectin-2 ELISA

Immobilized Human PVRIG, Fc Tag (Cat. No. PVG-H5257) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Nectin-2, Fc,Avitag (Cat. No. CD2-H82F8) with a linear range of 0.2-16 ng/mL (Routinely tested).

 Nectin-2 ELISA

Serial dilutions of TIGIT antibody antibody (1:2 serial dilutions, from 20 μg/mL to 0.0097 μg/mL) were added into Human TIGIT, Mouse IgG2a Fc Tag (Cat. No. TIT-H5253): Biotinylated Human Nectin-2, Fc,Avitag (Cat. No. CD2-H82F8) binding reactions. The assay was performed according to the above described protocol. Background was subtracted from data points before curve fitting.

  • Background
    Poliovirus receptor-related 2 (PVRL2) is also known as nectin-2 and CD112 (formerly herpesvirus entry mediator B, HVEB), which is a human plasma membrane glycoprotein. PVRL2 is one of the plasma membrane components of adherens junctions. PVRL2 also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in PVRL2 gene have been associated with differences in the severity of multiple sclerosis. Also, PVRL2 binds with low affinity to TIGIT. Furthermore, PVRL2 acts as a receptor for herpes simplex virus 1 (HHV-1) mutant Rid1, herpes simplex virus 1 (HHV-2) and pseudorabies virus (PRV).
  • Clinical and Translational Updates

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