Save Product
Product Details
Product Overview
Vascular endothelial growth factor (VEGF or VEGF-A) is a signaling protein that stimulates the formation of blood vessels (angiogenesis). There are several members of the VEGF family, with VEGF-A being the main and most extensively studied one. VEGF proteins function as homodimers (two identical subunits) linked by disulfide bonds. Each subunit is approximately 40–45 kDa, and the VEGF-A dimer binds to VEGF receptors (VEGFRs) on endothelial cells. Human VEGF ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human VEGF/VEGF-A dimers that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteVEGFFormat96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity<7 pg/mLRange31.25 pg/mL-2000 pg/mLAssay Time3 hr 10 minSample volume100 μLSample TypeCell Culture Supernatants, Plasma, Serum.NIBSC Code19/246Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
This kit is specifically designed for the accurate quantification of human VEGF/VEGF-A dimers in cell culture supernatants, serum, and plasma. The standard used is a recombinant VEGF dimer protein, and the antibodies specifically recognize the VEGF dimer.
It is for research use only.
Workflow
Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA068-C01Pre-coated Anti-VEGF Antibody Microplate1 plateCEA068-C02Human VEGF Standard2000 pg×2CEA068-C03Biotin-Anti-VEGF Antibody Con. Solution120 μLCEA068-C04Biotin-Antibody Dilution Buffer15 mLCEA068-C05Streptavidin-HRP Con. Solution120 μLCEA068-C06Streptavidin-HRP Dilution Buffer15 mLCEA068-C0720×Washing Buffer25 mLCEA068-C08Sample Dilution Buffer15 mL×2CEA068-C09Substrate Solution15 mLCEA068-C10Stop Solution15 mLACRO Quality Management System
Customers Also Viewed
Performance Data
Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of VEGF is less than 7 pg/mL.
Sample Values
Serum Sample: Twenty-four healthy serum donors were evaluated for the concentrations of human VEGF in assay.
Intra-Assay Statistics
Ten replicates of each of three samples containing different VEGF concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Inter-Assay Statistics
Three samples containing different concentrations of VEGF were tested in independent assays. Acceptable criteria: CV<15%.
Recovery
Recombinant VEGF was spiked into three human serum samples, and then analyzed. The average recovery of VEGF for serum samples is 85.87%.
Customer Reviews Writing Reviews
Recent Advances
Contact Us
[North America]: +1 800-810-0816
[Switzerland]: +41 800 040 012
[Asia & Pacific]: +86 400-682-2521
Drug Candidate Licensing