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Product Details
Product Overview
Human PSMA/FOLH1 ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human PSMA that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalytePSMA/FOLH1Format96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity2 ng/mLAssay Time2.75 hrSample volume50 μLRange3.125 ng/mL-200 ng/mLSample TypeFor the quantitative determination of human PSMA in serum, plasma and cell supernatant.Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant human PSMA in serum, plasma and cell culture supernatants.
It is for research use only.
Workflow
Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA242-C01Pre-coated Anti-PSMA Antibody Microplate1 plateCEA242-C02Human PSMA Standard40 μg×2CEA242-C03Biotin-Anti-PSMA Antibody Con. Solution100 μLCEA242-C04Biotin-Antibody Dilution Buffer8 mLCEA242-C05Streptavidin-HRP Con. Solution500 μLCEA242-C06Streptavidin-HRP Dilution Buffer15 mLCEA242-C0720×Washing Buffer50 mLCEA242-C08Sample Dilution Buffer15 mL×2CEA242-C09Substrate Solution12 mLCEA242-C10Stop Solution6 mLACRO Quality Management System
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Performance Data
Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of PSMA is less than 2 ng/mL.
Sample Values
Serum Sample: Twenty-four healthy serum samples were evaluated for the concentrations of human PSMA in assay.
Intra-Assay Statistics
Ten replicates of each of three samples containing different PSMA concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Inter-Assay Statistics
Three samples containing different concentrations of PSMA were tested in independent assays. Acceptable criteria: CV<15%.
Recovery
Recombinant PSMA was spiked into three human serum samples, and then analyzed. The average recovery of PSMA for serum samples is 100.5%.
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