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Human TIGIT Protein, Fc Tag (MALS verified)

COADS MS
DS

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Cat. No. / Size
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TIT-H5254-100ug
$415.00
TIT-H5254-1mg (250ug X 4)
$2615.00
ETA of in-stock products:2 business days
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Product Details

  • Synonym

    TIGIT, VSIG9, VSTM3

  • Source

    Human TIGIT, Fc Tag (TIT-H5254) is expressed from human 293 cells (HEK293). It contains AA Met 22 - Pro 141 (Accession # Q495A1-1).

    Predicted N-terminus: Met 22

    Request for sequence

  • Molecular Characterization

    TIGIT Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 39.7 kDa. The protein migrates as 45-54 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    TIGIT SDS-PAGE

    Human TIGIT, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    TIGIT SEC-MALS

    The purity of Human TIGIT, Fc Tag (Cat. No. TIT-H5254) is more than 95% and the molecular weight of this protein is around 85-100 kDa verified by SEC-MALS.

    Report

  • Bioactivity-ELISA

    TIGIT ELISA

    Immobilized Human CD155, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD5-H5254) at 5 μg/mL (100 μL/well) can bind Human TIGIT, Fc Tag (Cat. No. TIT-H5254) with a linear range of 0.6-10 ng/mL (QC tested).

    Protocol

  • TIGIT ELISA

    Serial dilutions of Human TIGIT Neutralizing antibody were added into Human TIGIT, Fc Tag (Cat. No. TIT-H5254): Biotinylated Human CD155, Fc,Avitag (Cat. No. CD5-H82F6) binding reactions. The half maximal inhibitory concentration (IC50) is 0.55834 μg/mL (Routinely tested).

    Protocol

  • Bioactivity-BLI

    TIGIT BLI

    Loaded Human TIGIT, Fc Tag (Cat. No. TIT-H5254) on Protein A Biosensor, can bind Human CD155, His Tag (Cat. No. CD5-H5223) with an affinity constant of 0.98 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

    Protocol

  • Bioactivity-FACS

    TIGIT FACS

    FACS assay shows that recombinant Human TIGIT, Fc Tag (Cat. No. TIT-H5254) can bind to 293T cell overexpressing human CD155. The concentration of TIGIT used is 0.1 μg/mL (Routinely tested).

    Protocol

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Background

T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.

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Drug Development Progress
  • English Name：

    T cell immunoglobulin and ITIM domains

  • Category：

  • Approved Drugs：

    0 Details

  • Drugs in Clinical Trials：

    38 Details

  • Highest Development Stage：

    Phase 3 Clinical

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