Product Details
Synonym
ROR1, NTRKR1
Source
Human / Cynomolgus / Rhesus macaque ROR1, Fc Tag (RO1-H5250) is expressed from human 293 cells (HEK293). It contains AA Gln 30 - Glu 403 (Accession # Q01973-1). In the region Gln 30 - Glu 403, the AA sequence of Human, Cynomolgus and Rhesus macaque ROR1 are homologus.
Predicted N-terminus: Gln 30
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 68.6 kDa. The protein migrates as 79-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Human / Cynomolgus / Rhesus macaque ROR1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS

The purity of Human / Cynomolgus / Rhesus macaque ROR1, Fc Tag (Cat. No. RO1-H5250) is more than 95% and the molecular weight of this protein is around 155-185 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Human / Cynomolgus / Rhesus macaque ROR1, Fc Tag (Cat. No. RO1-H5250) at 2 μg/mL (100 μL/well) can bind Anti-ROR1 Antibody, Human IgG1 with a linear range of 0.3-5 ng/mL (QC tested).
Protocol
Bioactivity-BLI

Loaded Anti-Human ROR1 MAb (Mouse IgG1) on AMC Biosensor, can bind Human / Cynomolgus / Rhesus macaque ROR1, Fc Tag (Cat. No. RO1-H5250) with an affinity constant of 0.357 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
Recent Advances
- English Name:
Inactive tyrosine-protein kinase transmembrane receptor ROR1
- Category:
- Approved Drugs:
0 Details
- Drugs in Clinical Trials:
13 Details
- Highest Development Stage:
Phase 3 Clinical
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