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Biotinylated Human PD-L1 / B7-H1 Protein, Fc,Avitag™,His Tag (MALS verified)

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PD1-H82F3-25ug
$395.00
PD1-H82F3-200ug
$1720.00
ETA of in-stock products:2 business days
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Product Details

  • Synonym

    PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1

  • Source

    Biotinylated Human PD-L1, Fc,Avitag,His Tag (PD1-H82F3) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1).

    Predicted N-terminus: Phe 19

    Request for sequence

  • Molecular Characterization

    PD-L1 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc fragment at the C-terminus, followed by a polyhistidine tag. The Avi tag (Avitag™) is inserted in-between the Fc and his tags. The protein has a calculated MW of 54.0 kDa. The protein migrates as 65-80 kDa on a SDS-PAGE gel when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    PD-L1 SDS-PAGE

    Biotinylated Human PD-L1, Fc,Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    PD-L1 SEC-MALS

    The purity of Biotinylated Human PD-L1, Fc,Avitag,His Tag (Cat. No. PD1-H82F3) is more than 95% and the molecular weight of this protein is around 120-135 kDa verified by SEC-MALS.

    Report

  • Bioactivity-ELISA

    PD-L1 ELISA

    Immobilized Human PD-1, Fc Tag, low endotoxin (HPLC-verified) (Cat. No. PD1-H5257) at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-L1, Fc,Avitag,His Tag (Cat. No. PD1-H82F3) with a linear range of 2-39 ng/mL (QC tested).

    Protocol

  • PD-L1 ELISA

    Immobilized Human B7-1, mouse IgG2a Fc Tag, low endotoxin (Cat. No. B71-H52A4) at 5 μg/mL (100 μL/well) can bind Biotinylated Human PD-L1, Fc,Avitag,His Tag (Cat. No. PD1-H82F3) with a linear range of 0.02-0.625 μg/mL (Routinely tested).

    Protocol

  • PD-L1 ELISA

    Serial dilutions of nivolumab were added into Human PD-1, Fc Tag, low endotoxin (HPLC-verified) (Cat. No. PD1-H5257): Biotinylated Human PD-L1, Fc,Avitag,His Tag (Cat. No. PD1-H82F3) binding reactions. The half maximal inhibitory concentration (IC50) is 0.5381 μg/mL (Routinely tested).

    Protocol

  • Bioactivity-FACS

    PD-L1 FACS

    Flow Cytometry assay shows that Biotinylated Human PD-L1, Fc,Avitag,His Tag (Cat.No. PD1-H82F3) can bind to 293 cell overexpressing human PD-1. The concentration of PD-L1 used is 1 μg/mL (Routinely tested).

    Protocol

  • PD-L1 FACS

    FACS analysis shows that the binding of Biotinylated Human PD-L1, Fc,Avitag,His Tag (Cat. No. PD1-H82F3) to 293 overexpressing PD-1 was inhibited by increasing concentration of neutralizing anti-PD-1 antibody. The concentration of PD-L1 used is 2 μg/mL. The IC50 is 0.64 μg/mL (Routinely tested).

    Protocol

  • Bioactivity-CELL BASE

    PD-L1 CELL

    Immobilized cell surface PD-1 (5x104 of cells per well) can bind Biotinylated Human PD-L1, Fc,Avitag,His Tag (Cat. No. PD1-H82F3) with an EC50 of 0.082 μg/mL (Routinely tested).

    Protocol

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Background

Programmed cell death 1 ligand 1 (PDL1) is also known as B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1 and PDCD1LG1,which is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses.PDL1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. This protein is broadly expressed in the majority of peripheral tissues as well as hematopoietic cells. Interaction between PDL1 and its receptors belonging to the CD28 family of molecules provide both stimulatory and inhibitory signals in regulating T cell activation and tolerance. PDL1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression.

Recent Advances

 
Drug Development Progress
  • English Name：

    Programmed death-ligand 1

  • Category：

  • Approved Drugs：

    11 Details

  • Drugs in Clinical Trials：

    152 Details

  • Highest Development Stage：

    Approved

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