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Product Details
Synonym
PDCD1, PD1, CD279, SLEB2
Source
Biotinylated Human PD-1 Protein, Avitag,His Tag (PD1-H82E4) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # Q15116-1).
Predicted N-terminus: Leu 25
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 18.6 kDa. The protein migrates as 35-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Application
PD1-H82E4 works best for experiments that test the binding between PD-1 and candidate antibodies, such as biopanning and other relevant assays.
This product is NOT suitable for testing PD1-PDL1 binding by ELISA. For this type of application, we strongly recommend you to choose PD1-H82F1 as an alternative.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Biotinylated Human PD-1 Protein, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human PD-1 Protein, Avitag,His Tag (Cat. No. PD1-H82E4) is more than 95% and the molecular weight of this protein is around 32-45 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Nivolumab at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-1 Protein, Avitag,His Tag (Cat. No. PD1-H82E4) with a linear range of 0.8-13 ng/mL (QC tested).
Protocol
Immobilized Human VEGF165, His Tag (Cat. No. VE5-H5248) at 2 μg/mL, add increasing concentrations of Ivonescimab and then add Biotinylated Human PD-1 Protein, Avitag,His Tag (Cat. No. PD1-H82E4) at 2 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) with sensitivity of 2.17 ng/mL (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Human PD-1 Protein, Avitag,His Tag (Cat. No. PD1-H82E4) on SA Biosensor, can bind Human PD-L1, His Tag (Cat. No. PD1-H5229) with an affinity constant of 2.4 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Biotinylated Human PD-1 Protein, Avitag,His Tag (Cat. No. PD1-H82E4) on SA Biosensor, can bind Human PD-L2 Protein, His Tag (Cat. No. PD2-H5220) with an affinity constant of 0.6 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Programmed cell death protein 1
- Category：
- Approved Drugs：
20 Details
- Drugs in Clinical Trials：
167 Details
- Highest Development Stage：
Phase 3 Clinical
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