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Human Noggin Protein, Fc Tag, premium grade

COADS MS
DS

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Cat. No. / Size
Price
Qty
NON-H5257-20ug
$105.00
NON-H5257-100ug
$185.00
NON-H5257-1mg (500ug X 2)
$1030.00
ETA of in-stock products:2 business days
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Product Details

  • Synonym

    Noggin, NOG, SYM1, SYNS1

  • Source

    Human Noggin Protein, Fc Tag, premium grade (NON-H5257) is expressed from human 293 cells (HEK293). It contains AA Gln 28 - Cys 232 (Accession # Q13253-1).

    Predicted N-terminus: Gln 28

    It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.

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  • Molecular Characterization

    Noggin Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 49.5 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.1 EU per μg by the LAL method / rFC method.

  • Sterility

    Negative

  • Mycoplasma

    Negative

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Noggin SDS-PAGE

    Human Noggin Protein, Fc Tag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • Bioactivity-Organoid Culture

    Noggin ORGANOID CULTURE

    Noggin (Cat. No. NON-H5257) maintains intestinal organoid growth well through multiple passages and long-term culture. The long-term cultivation effect is significantly better than that of competing companies. The organoids showed good budding morphology.

  • Noggin ORGANOID CULTURE

    Human EGF (Cat. No. EGF-H52H3), Noggin (Cat. No. NON-H5257), R-spondin1 (Cat. No. RS6-H4220), FGF7 (Cat. No. FG7-H52H5), FGF10, HGF (Cat. No. HGF-H52H3) actively support liver ductal organoid growth.

  • Bioactivity-CELL BASE

    Noggin CELL

    Human Noggin Protein, Fc Tag, premium grade (Cat. No. NON-H5257) inhibits the activation of Human BMP (Luc) HEK 293 Reporter cells by Human BMP-4 Protein. The typical EC50 for this effect is 233.5 ng/mL (QC tested).

    Protocol

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Background

Noggin is also known as NOG，SYM1, SYNS1 and is a secreted homodimeric glycoprotein whose scaffold contains a cystine-knot topology similar to that of BMPs.Secreted Noggin probably remains close to the cell surface due to its binding of heparincontaining proteoglycans.Noggin inhibits TGF-β signal transduction by binding to TGF-β family ligands and preventing them from binding to their corresponding receptors. Noggin plays a key role in neural induction by inhibiting BMP4, along with other TGF-β signaling inhibitors such as chordin and follistatin. Mouse knockout experiments have demonstrated that noggin also plays a crucial role in bone development, joint formation, and neural tube fusion. During embryogenesis, Noggin antagonizes specific BMPs at defined times, for example, during neural tube, somite and cardiomyocyte growth and patterning. During skeletal development, Noggin prevents chondrocyte hyperplasia, thus allowing proper formation of joints. During culture of human embryonic stem cells (hESC) or neural stem cells under certain conditions, addition of Noggin to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state, or alternatively, to differentiate into dopaminergic neurons Noggin also appears to maintain adult stem cell populations in vivo, for example, maintaining neural stem cells within the hippocampus.

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  • Background