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Influenza A [Wisconsin/588/2019(H1N1)] Neuraminidase (NA) Protein, His Tag

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NEE-V524k-100ug
$360.00
NEE-V524k-1mg
$2760.00
ETA of in-stock products:2 business days
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Product Details

  • Synonym

    Neuraminidase/NA (Influenza Virus)

  • Source

    Influenza A [Wisconsin/588/2019(H1N1)] Neuraminidase (NA) Protein, His Tag (NEE-V524k) is expressed from human 293 cells (HEK293). It contains AA Val 80 - Lys 469 (Accession # EPI_ISL_404527, GISAID).

    Predicted N-terminus: His

    Request for sequence

  • Molecular Characterization

    Neuraminidase/NA (Influenza Virus) Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 50.8 kDa. The protein migrates as 60-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 50 mM Tris, 300 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Neuraminidase/NA (Influenza Virus) SDS-PAGE

    Influenza A [Wisconsin/588/2019(H1N1)] Neuraminidase (NA) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

    Neuraminidase/NA (Influenza Virus) ELISA

    Immobilized Influenza A [Wisconsin/588/2019(H1N1)] Neuraminidase (NA) Protein, His Tag (Cat. No. NEE-V524k) at 1 μg/mL (100 μL/well) can bind Anti-NA(A/PAN) ,Mouse IgG with a linear range of 0.2-4 ng/mL (QC tested).

    Protocol

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Background

Neuraminidase () and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme which facilitates virus release and efficient spread of the progeny virus from cell to cell.

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