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Product Details
Synonym
MSLN, Mesothelin, MPF
Source
Alexa Fluor 488-Labeled Human Mesothelin (296-580), His Tag (MSN-HA2H9) is produced via conjugation of AF488 to Human Mesothelin (296-580), His Tag with a new generation site-specific technology under Star Staining labeling platform. Human Mesothelin (296-580), His Tag is expressed from human 293 cells (HEK293). It contains AA Glu 296 - Gly 580 (Accession # AAH09272.1).
Predicted N-terminus: Glu 296
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
Conjugate
AF488
Excitation Wavelength: 488 nm
Emission Wavelength: 517 nm
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
Bioactivity-FACS
The activity of Alexa Fluor 488-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HA2H9) was higher than other Competitor.
Protocol
Evaluation of CAR expression
FACS Analysis of Anti-Mesothelin CAR Expression
5e5 of anti-MSLN CAR-293 cells were stained with 100 μL of 3 μg/mL of AF488-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HA2H9) and negative control protein respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). AF488 signal was used to evaluate the binding activity (QC tested).
ProtocolFACS Analysis of Non-specific binding to PBMCs
5e5 of PBMCs were stained with AF488-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HA2H9) and anti-CD3 antibody, washed and then analyzed with FACS. PE signal was used to evaluate the expression of CD3+ T cells in PBMCs, and AF488 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Mesothelin
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
41 Details
- Highest Development Stage：
Phase 2 Clinical
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