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Product Details
Synonym
GDNF, ATF
Source
Human GDNF Protein, premium grade (GDF-H5219) is expressed from human 293 cells (HEK293). It contains AA Ser 78 - Ile 211 (Accession # P39905-1).
Predicted N-terminus: Ser 78
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries no "tag".
The protein has a calculated MW of 15.1 kDa. The protein migrates as 15-22 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.1 EU per μg by the LAL method / rFC method.
Sterility
Negative
Mycoplasma
Negative
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Human GDNF Protein, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-Organoid Culture
iPSC derived human brain organoids, expressing neuron marker TUJ1 (green) and neuron stem cell marker NESTIN (red), were cultured for 36 days with BDNF (Cat. No. BDF-H5219) and GDNF (Cat. No. GDF-H5219). H&E staining showed brain organoids had classic neuron stem cell rossettes histology.
Bioactivity-Stem Cell Culture
iPSC derived neurons forming axon net-works were cultured with differentiation protocol, including BDNF (Cat. No. BDF-H5219) and GDNF (Cat. No. GDF-H5219).
Bioactivity-ELISA
Immobilized Human GDNF Protein, premium grade (Cat. No. GDF-H5219) at 2 μg/mL (100 μL/well) can bind Human GFR alpha-1, His Tag (Cat. No. GF1-H5224) with a linear range of 0.2-4 ng/mL (QC tested).
Protocol
Application Data
Human GDNF Protein, premium grade (Cat. No. GDF-H5219) combined with Human BDNF Protein (E. coli), premium grade (Cat. No. BDF-H5116) could efficiently induce iPSC differentiation to dopaminergic (DA) neurons, with high expression of DA markers (TH-1, MAP2) in immunofluorescence and FACS.
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Background
Recent Advances
Drug Development Progress- English Name：
Glial cell line-derived neurotrophic factors
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
4 Details
- Highest Development Stage：
NDA/BLA
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