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Product Details
Synonym
FOLR-1, FBP, FOLR, FRα
Source
Biotinylated Human FOLR1, His,Avitag (FO1-H82E2) is expressed from human 293 cells (HEK293). It contains AA Arg 25 - Met 233 (Accession # P15328-1).
Predicted N-terminus: Arg 25
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 28.3 kDa. The protein migrates as 35-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human FOLR1, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human FOLR1, His,Avitag (Cat. No. FO1-H82E2) is more than 95% and the molecular weight of this protein is around 35-45 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Folic acid-BSA conjugate at 5 μg/mL (100 μL/well) can bind Biotinylated Human FOLR1, His,Avitag (Cat. No. FO1-H82E2) with a linear range of 0.004-0.125 μg/mL (QC tested).
Protocol
Immobilized Mouse Anti-FOLR1 Antibody at 1 μg/mL (100 μL/well) can bind Biotinylated Human FOLR1, His,Avitag (Cat. No. FO1-H82E2) with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Biotinylated Human FOLR1, His,Avitag (Cat. No. FO1-H82E2) immobilized on SA Chip can bind Folic acid-BSA with an affinity constant of 83.8 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-FACS
2e5 of anti-FOLR1 CAR-293 cells were stained with 100 μL of 1 μg/mL of Biotinylated Human FOLR1, His,Avitag (Cat. No. FO1-H82E2) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).
Protocol
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Background
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- Category：
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- Highest Development Stage：
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