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Product Details
Synonym
CD8A, CD8, Leu2, MAL, p32
Source
Alexa Fluor 488-Labeled Human CD8 alpha Protein, His Tag (CDA-HA2H6) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Asp 182 (Accession # P01732-1). It is the Alexa Fluor 488 labeled form of Human CD8 alpha Protein, His Tag.
Predicted N-terminus: Ser 22
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 19.5 kDa. The protein migrates as 23-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Conjugate
AF488
Excitation Wavelength: 488 nm
Emission Wavelength: 517 nm
Labeling
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with AF488 using standard chemical labeling method. The residual AF488 is removed by molecular sieve treatment during purification process.
Protein Ratio
The AF488 to protein molar ratio is 1-3.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Alexa Fluor 488-Labeled Human CD8 alpha Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Alexa Fluor 488-Labeled Human CD8 alpha Protein, His Tag (Cat. No. CDA-HA2H6) at 5 μg/mL (100 μL/well) can bind Anti-CD8 alpha Antibody, Human IgG1 with a linear range of 0.2-16 ng/mL (Routinely tested).
Protocol
Immobilized Anti-CD8 alpha Antibody, Human IgG1 at 5 μg/mL (100 μL/well) can bind Alexa Fluor 488-Labeled Human CD8 alpha Protein, His Tag (Cat. No. CDA-HA2H6) with a linear range of 1-16 ng/mL (Routinely tested).
Protocol
Bioactivity-FACS
1e5 of Mouse Anti-CD8 antibody coupled beads (5.5 μm) were stained with different concentration of Alexa Fluor 488-Labeled Human CD8 alpha Protein, His Tag (Cat. No. CDA-HA2H6) and negative control protein respectively, AF488 signal was used to evaluate the binding activity (QC tested).
Protocol
1e5 of Mouse Anti-CD8 antibody coupled beads (5.5 μm) were stained with different concentration of Alexa Fluor 488-Labeled Human CD8 alpha Protein, His Tag (Cat. No. CDA-HA2H6) and negative control protein respectively, AF488 signal was used to evaluate the binding activity (QC tested).
Protocol
Bioactivity-Stability
Binding activity of three different lots of Alexa Fluor 488-Labeled Human CD8 alpha Protein, His Tag against Mouse Anti-CD8 antibody coupled beads (5.5 μm) was evaluated by flow cytometry. The result shows very high batch-to-batch consistency.
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Background
Recent Advances
Drug Development Progress- English Name：
Cluster of differentiation 8
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
15 Details
- Highest Development Stage：
Phase 3 Clinical
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