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Product Details
Synonym
CD4, CD4mut, LEU3
Source
Alexa Fluor 488-Labeled Human CD4 Protein, His Tag (CD4-HA2H7) is produced via conjugation of AF488 to Human CD4 Protein, His Tag with a new generation site-specific technology under Star Staining labeling platform. Human CD4 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Pro 396 (Accession # AAH25782).
Predicted N-terminus: Lys 26
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
Conjugate
AF488
Excitation Wavelength: 488 nm
Emission Wavelength: 517 nm
Protein Ratio
The AF488 to protein molar ratio is 0.9-1.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
Bioactivity-ELISA
Immobilized Anti-CD4 Antibody (Ibalizumab-IgG4) at 1 μg/mL (100 μL/well) can bind Alexa Fluor 488-Labeled Human CD4 Protein, His Tag (Cat. No. CD4-HA2H7) with a linear range of 0.2-16 ng/mL (Routinely tested). Labeling with fluorescent dyes did not affect their activity.
Protocol
Bioactivity-FACS
1e5 of Mouse Anti-CD4 antibody coupled beads (5.5 μm) were stained with different concentration of Alexa Fluor 488-Labeled Human CD4 Protein, His Tag (Cat. No. CD4-HA2H7) and negative control protein respectively, AF488 signal was used to evaluate the binding activity (QC tested).
Protocol
1e5 of Mouse Anti-CD4 antibody coupled beads (5.5 μm) were stained with different concentration of Alexa Fluor 488-Labeled Human CD4 Protein, His Tag (Cat. No. CD4-HA2H7) and negative control protein respectively, AF488 signal was used to evaluate the binding activity (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
T-cell surface antigen CD4
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
22 Details
- Highest Development Stage：
Approved
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