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Product Details
Synonym
TNFSF13B, BAFF, BLYS, CD257, DTL, TALL1, THANK, TNFSF20, ZTNF4, TALL-1
Source
Human BAFF Protein, Fc Tag (BAF-H5261) is expressed from human 293 cells (HEK293). It contains AA Glu 140 - Leu 285 (Accession # Q9Y275-1) trimer Design.
Predicted N-terminus: Pro
Molecular Characterization
This protein carries a human IgG1 Fc tag at the N-terminus.
The protein has a calculated MW of 76.7 kDa. The protein migrates as >170 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.1 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human BAFF Protein, Fc Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human BAFF Protein, Fc Tag (Cat. No. BAF-H5261) is more than 90% and the molecular weight of this protein is around 150-180 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human BCMA, Mouse IgG2a Fc Tag (Cat. No. BCA-H5253) at 1 μg/mL (100 μL/well) can bind Human BAFF Protein, Fc Tag (Cat. No. BAF-H5261) with a linear range of 0.2-6 ng/mL (QC tested).
Protocol
Immobilized Human BAFF Protein, Fc Tag (Cat. No. BAF-H5261) at 1 μg/mL (100 μL/well) can bind Biotinylated Human TACI, Fc,Avitag (Cat. No. TAI-H82F6) with a linear range of 0.002-0.05 μg/mL (Routinely tested).
Protocol
Immobilized Human BAFF Protein, Fc Tag (Cat. No. BAF-H5261) at 1 μg/mL (100 μL/well) can bind Biotinylated Mouse BAFFR, Fc,Avitag (Cat. No. BAR-M82F0) with a linear range of 0.4-25 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Immobilized Human BAFF Protein, Fc Tag (Cat. No. BAF-H5261) on CM5 Chip can bind Human TACI Protein, Fc Tag with an affinity constant of 0.658 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Immobilized Human BAFF Protein, Fc Tag (Cat. No. BAF-H5261) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human BAFFR Protein, Llama IgG2b Fc Tag (Cat. No. BAR-H5258) with an affinity constant of 44.5 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human BAFF Protein, Fc Tag (Cat. No. BAF-H5261) on Protein A Biosensor, can bind Biotinylated Human BCMA, His,Avitag (Cat. No. BCA-H82E4) with an affinity constant of 0.69 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Bioactivity-CELL BASE
Human BAFF Protein, Fc Tag (Cat. No. BAF-H5261) stimulates proliferation of mouse spleen cells. The EC50 for this effect is 4.91-9.81 ng/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
B-cell-activating factor/B lymphocyte stimulator
- Category：
- Approved Drugs：
2 Details
- Drugs in Clinical Trials：
8 Details
- Highest Development Stage：
Approved
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