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Product Details
Product Overview
Human MUCIN 1/MUC1 ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant humanMUCIN 1/MUC1 that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteMUCIN 1 / MUC1Format96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity20 pg/mLAssay Time2.75 hrSample volume50 μLRange31.25 pg/mL-2000 pg/mLSample TypeFor the quantitative determination of human MUCIN 1/MUC1 in serum, plasma and cell supernatant.Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant human MUCIN 1/MUC1 in serum, plasma and cell culture supernatants.
It is for research use only.
Workflow
Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA055-C01Pre-coated Anti-MUC1 Antibody Microplate1 plateCEA055-C02Human MUC1 Standard100 ng×2CEA055-C03Biotin-Anti-MUC1 Antibody Con. Solution100 μLCEA055-C04Biotin-Antibody Dilution Buffer8 mLCEA055-C05Streptavidin-HRP Con. Solution500 μLCEA055-C06Streptavidin-HRP Dilution Buffer15 mLCEA055-C0720×Washing Buffer50 mLCEA055-C08Sample Dilution Buffer15 mL×2CEA055-C09Substrate Solution12 mLCEA055-C10Stop Solution6 mLACRO Quality Management System
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Performance Data
Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was cal-culated based on the results of the standard curve. The minimum detectable con-centration of MUC1 is less than 20 pg/mL.
Sample Values
Serum Sample: MUC1 concentrations were measured in forty samples provided by human donors, which were collected from healthy human donors. The measurements of forty samples are in the range of 248-4600 ng/mL and shown in the figure below.
Intra-Assay Statistics
Ten replicates of each of three samples containing different MUC1 concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Inter-Assay Statistics
Three samples containing different concentrations of MUC1 were tested in inde-pendent assays. Acceptable criteria: CV<15%.
Recovery
Recombinant MUC1 was spiked into three human serum samples, and then ana-lyzed. The average recovery of MUC1 for serum samples is 101.6%.
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Drug Development Progress- English Name：
Mucin-1
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
22 Details
- Highest Development Stage：
Phase 2 Clinical
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