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Product Details
Product Specifications
Assay TypeSandwich-ELISAAnalytePyrophosphataseFormat96T (8×12 strips)ReactivityHumanRegulatory StatusRUOSensitivity< 9.38 pg/mLStandard Curve Range9.375 pg/mL-300 pg/mLAssay Time3 hr 20 minSuitable Sample TypeFor the quantitative determination of Pyrophosphatase in Cell Culture Supernatants.Sample volume100 μLProduct Overview
resDetect™ Pyrophosphatase ELISA Kit is based on the ELISA sandwich method and is used to detect and quantitatively determine Pyrophosphatase residues in mRNA drug products or semi-manufactures. It contains Pyrophosphatase Standard (ACROBiosystems, cat# PYE-T5143) to ensure accurate assay results and is designed to provide a reliable solution for monitoring and controlling Pyrophosphatase residues during the production of mRNA preparations. It can also be used as a universal detection tool for the quantitative determination of Pyrophosphatase.
Reconstitution
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
Storage
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeRES005-C01Pre-coated Anti-Pyrophosphatase Antibody Microplate1 plate (8×12 strips)RES005-C02Pyrophosphatase Standard100 μLRES005-C03Biotin-Anti-Pyrophosphatase Antibody15 μgRES005-C04Streptavidin-HRP50 μLRES005-C0520xWashing Buffer50 mLRES005-C062xDilution Buffer50 mLRES005-C07Substrate Solution12 mLRES005-C08Stop Solution7 mLACRO Quality Management System
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Performance Data
Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
Intra-Assay Statistics
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision, Intra-Assay Precision CV＜15%.
Inter-Assay Statistics
Three samples of known concentration were tested in three separate assays to assess inter-assay precision, Inter-Assay Precision CV<15%.
Recovery
Three Pyrophosphatase with different concentrations were tested to calculate the recovery rate.
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Pyrophosphatases
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