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Materials Provided
IDComponentsSizeEP101-C01High-bind Plate1 plateEP101-C02Human PD-L125 μgEP101-C03Anti-PD-1 Neutralizing Antibody20 μgEP101-C04Human PD-1-Biotin5 μgEP101-C05Streptavidin-HRP10 μgEP101-C06Coating Buffer12 mLEP101-C0710xWashing Buffer50 mLEP101-C08Blocking Buffer50 mLEP101-C09Substrate Solution12 mLEP101-C10Stop Solution7 mLProduct Overview
PD-1 [Biotinylated] : PD-L1 Inhibitor Screening ELISA Kit is based on a competition-ELISA method, is used to screening for inhibitors of human PD-1 binding to human PD-L1. The kit is designed to provide a reliable solution for the screening of antibodies and small molecule inhibitors in the early stages, as well as QC release, to help researchers quickly screen and validate drugs, effectively improving research and development efficiency. It can also be used as a universal detection tool to identify the ability of human PD-1 binding to human PD-L1.
Reconstitution
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
Storage
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
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Performance Data
Typical Data
Please refer to DS document for the assay protocol.
INHIBITION OF PD-1-PD-L1 BINDING BY ANTI-PD-1 NEUTRALIZING ANTIBODY (CATALOG # EP101-C03) MEASURED USING THE PD-1 [BIOTINYLATED] : PD-L1 INHIBITOR SCREENING ELISA ASSAY PAIR (CATALOG # EP-101).
Anti-PD-1 neutralizing antibody was diluted from 10 μg/mL to 0.078 μg/ mL (69.628 nM to 0.544 nM) and loaded onto the plate coated by human PD-L1 in the presence of human PD-1-Biotin. Assay was performed according to the protocol in PD-1[Biotinylated] : PD-L1 Inhibitor Screening ELISA Assay Pair. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
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