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Product Details
Product Overview
Human IFN-gamma Quick ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human IFN-gamma that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteIFN-γFormat96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity20 pg/mLAssay Time1.75 hrSample volume50 μLRange54.7 pg/mL-3500 pg/mLSample TypeCell Culture Supernatants, Plasma, Serum.NIBSC Code87/586Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant human IFN-gamma in serum, plasma and cell culture supernatants.
It is suitable for potency assay of mAbs, CAR-T/NK cell therapy.
This Assay has been anchored to NlBSC/WHO standards (Code: 87/586), ELISA Kit: NIBSC/WHO Mass Conversion Factor is 1.
It is for research use only.
Workflow
Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA245-C01Pre-coated Anti-IFN-γ Antibody Microplate1 plate (8×12 strips)CEA245-C02Human IFN-γ Standard35 ng×2CEA245-C03Biotin-Anti-IFN-γ Antibody25 μgCEA245-C04Biotin-Antibody Dilution Buffer8 mLCEA245-C05Streptavidin-HRP Con. Solution500 μLCEA245-C06Streptavidin-HRP Dilution Buffer15 mLCEA245-C0720×Washing Buffer50 mLCEA245-C08Sample Dilution Buffer15 mL×2CEA245-C09Substrate Solution12 mLCEA245-C10Stop Solution6 mLACRO Quality Management System
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Performance Data
Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was cal-culated based on the results of the standard curve. The minimum detectable con-centration of IFN-γ is less than 20 pg/mL.
Sample Values
Serum Sample: Forty healthy serum samples were evaluated for the concentrations of human IFN-γ in assay.
Cell Culture Supernatant: Human peripheral blood mononuclear cells (PBMCs) obtained were plated at a density of 1 × 10⁶ cells/mL in complete RPMI 1640 medium containing 10% fetal bovine serum, 2 mM L-glutamine, and antibiotic supplements (penicillin 100 U/mL with streptomycin sulfate 100 μg/mL). Following a 5-day incubation period, experimental groups were either maintained as untreated controls or stimulated with 10 μg/mL phytohemagglutinin (PHA). Subsequent analysis involved quantitative measurement of IFN-gamma concentrations in collected culture supernatants using standardized immunoassays.
Intra-Assay Statistics
Ten replicates of each of three samples containing different IFN-γ concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Inter-Assay Statistics
Three samples containing different concentrations of IFN-γ were tested in independent assays. Acceptable criteria: CV<15%.
Recovery
Recombinant IFN-γ was spiked into three human serum samples, and then analyzed. The average recovery of IFN-γ for serum samples is 108.9%.
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Recent Advances
Drug Development Progress- English Name：
Interferon gamma
- Category：
- Approved Drugs：
3 Details
- Drugs in Clinical Trials：
12 Details
- Highest Development Stage：
Approved
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