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Double-Stranded RNA (dsRNA) ELISA Kit

COADSMS
DS

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RES-A092-96tests
$860.00
ETA of in-stock products:2 business days
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Product Details

  • FAB

    1. High Sensitivity - Detects dsRNA at as low as 8.988 pg/mL, crucial for trace dsRNA detection in mRNA vaccine QC.
    2. High Specificity - Specifically identifies dsRNA, not cross - reacting with ssRNA or dsDNA for accurate detection.
    3. Broad Applicability - Recognizes >40bp dsRNA, including various modified types, suitable for diverse scenarios.
    4. Flexible usage - Customers can purchase 5-OMe-UTP dsRNA, N1-Me-pUTP dsRNA, and pUTP dsRNA standard according to their own needs.
    5. Good Precision and Accuracy - High intra-assay as well as inter-assay precision, and accurate recovery for high, middle, low-concentration samples to ensure reliable results.

  • Product Specifications

    Assay Type
    Sandwich-ELISA
    Analyte
    dsRNA
    Format
    96T (8×12 strips)
    Reactivity
    /
    Regulatory Status
    RUO
    Sensitivity
    8.988 pg/mL
    Standard Curve Range
    25 pg/mL-800 pg/mL
    Assay Time
    2 hr 20 min
    Suitable Sample Type
    For the detection and quantitative determination of dsRNA in samples
    Sample volume
    100 μL

  • Product Overview

    Double-Stranded RNA (dsRNA) ELISA Kit is based on the Sandwich-ELISA method and is used for the rapid detection and quantitative determination of dsRNA concentrations in mRNA vaccine formulations. It is designed to precisely detect and control the amount of dsRNA in mRNA vaccines, which is crucial for ensuring vaccine quality and safety.

  • Application

    1.mRNA Vaccine Development

    2.Quality Control of IVT Products

    3.Research on Viral Infections

    It is for research use only.

  • Reconstitution

    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

  • Storage

    1.Unopened kit should be stored at 2℃-8℃ upon receiving.

    2.Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3.The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Materials Provided

    ID
    Components
    Size
    RES092-C01
    Pre-coated Anti-dsRNA Monoclonal Antibody (K1) Microplate
    1 plate (8×12 strips)
    RES092-C02
    Unmodified dsRNA (400bp) Standard
    10 μg×2
    RES092-C03
    HRP-Anti-dsRNA Monoclonal Antibody (J2)
    10 μg×2
    RES092-C04
    10×Washing Buffer
    50 mL
    RES092-C05
    Sample Dilution Buffer
    50 mL
    RES092-C06
    HRP-Antibody (J2) Dilution Buffer
    25 mL
    RES092-C07
    Substrate Solution
    12 mL
    RES092-C08
    Stop Solution
    7 mL

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Typical Data 

    Please refer to DS document for the assay protocol.

    dsRNA TYPICAL DATA

    For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.

Validation

  • Intra-Assay Statistics

    Three samples of known concentration were tested ten times on one plate to assess intra-assay precision.

    dsRNA INTRA-ASSAY STATISTICS

  • Inter-Assay Statistics

    Three samples of known concentration were tested in three separate assays to assess inter-assay precision.

    dsRNA INTER-ASSAY STATISTICS

  • Recovery

    Three samples with different concentrations were tested to calculate the recovery rate.

    dsRNA RECOVERY

  • Specificity

    The kit has been tested to specifically identify dsRNA, and do not identify ssRNA , dsDNA.

    dsRNA SPECIFICITY

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