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resDetect™ Vero resDNA Quantitation Kit (qPCR)

COADSMS
DS

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Cat. No. / Size
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OPA-R018-100tests
$1650.00
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Product Details

  • Product Overview

    Vero resDNA Quantitation Kit is designed for quantitative detection of residual Vero DNA in biopharmaceutical productions (Antibodies, cells, viruses, etc.). Based on real-time quantitative PCR (qPCR) method, this kit makes the detection of the residual DNA from Vero cells rapid and reliable. The lower limit of quantitation is 3 fg/µL. All procedures are typically in less than 4 hours.

    Use this kit to detect residual DNA after the DNA is extracted from test samples. For achieving the better DNA recovery, it is recommended to use the resDetect™ resDNA Sample Preparation Kit Ⅱ(Magnetic Beads) (Cat. No. OPA-R024) in combination.

  • Features

    1. High sensitivity for optimal product safety: LLOD (1 fg/µL) for detection of residue host cell DNA
    2. High specificity: No cross-reactivity with unrelated DNA
    3. Validation: ICH Q2(R2) as Validation of Analytical Procedures
    4. High-quality: This kit is manufactured in ISO 13485 standard facility.

  • Application

    The kit is used for quantitative detection of residual Vero DNA in biopharmaceutical production.

    Suitable for manufacturing process or quality control inspection.

    It is for research use only.

  • Technical Specifications

    DNA Technical Specifications

  • Attention

    If your experimental process requires sample preparation, please purchase and conduct the experiment using the sample preparation kits recommended in the table to ensure that the buffers used in both the sample preparation and resDNA detection are consistent.

    DNA Attention

  • Materials Provided

    ID
    Components
    Size
    OPA-R018-01
    2×qPCR Master Mix
    1.0 mL×2
    OPA-R018-02
    Vero Primer & Probe Mix
    700 μL
    OPA-R018-03
    Vero DNA Control（3ng/μL）
    100 μL
    OPA-R018-04
    Dilution Buffer
    1.5 mL×3
    OPA-R018-05
    DNase/RNase-free Water
    1.0 mL

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Typical Data 

    Please refer to DS document for the assay protocol.

    DNA TYPICAL DATA

    Figure 1. High sensitivity and broad dynamic range using the Vero resDNA Quantitation Kit. (A) Typical analysis results obtained with Standard 1 (30 pg/μL) to 5 (3 fg/μL). (B) The standard curve of the 10-fold dilution series. PCR efficiency should be 90-110%.

  • DNA TYPICAL DATA

    Figure 2. Assay specificity. Standard curves generated using 10-fold serial dilution of Vero genomic DNA (included in the kit) in the presence of E. coli DNA, CHO DNA and Pichia DNA.

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