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Product Details
Product Overview
Human HGFR/c-MET ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural soluble human HGFR/c-MET that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalytesoluble HGFR / c-METFormat96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity70 pg/mLAssay Time2.75 hrSample volume50 μL (10 μL serum)Range125 pg/mL-8000 pg/mlSample TypeFor the quantitative determination of human soluble HGFR/c-MET in serum, plasma and cell supernatant.Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of soluble human HGFR/c-MET in serum and plasma.
It is for research use only.
Workflow
Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA073-C01Pre-coated Anti-c-MET Antibody Microplate1 plateCEA073-C02Human c-MET Standard0.4 μg×2CEA073-C03Biotin-Anti-c-MET Antibody Con. Solution150 μLCEA073-C04Biotin-Antibody Dilution Buffer8 mLCEA073-C05Streptavidin-HRP Con. Solution500 μLCEA073-C06Streptavidin-HRP Dilution Buffer15 mLCEA073-C0720×Washing Buffer50 mLCEA073-C08Sample Dilution Buffer15 mL×2CEA073-C09Substrate Solution12 mLCEA073-C10Stop Solution6 mLACRO Quality Management System
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Performance Data
Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was cal-culated based on the results of the standard curve. The minimum detectable con-centration of c-MET is less than 70 pg/mL.
Sample Values
Serum Sample: c-MET concentrations were measured in forty human serum samples, which were collected from healthy human subiects. The measurements of forty samples are in the range of 107-415 ng/mL and shown in the figure below.
Intra-Assay Statistics
Ten replicates of each of three samples containing different c-MET concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Inter-Assay Statistics
Three samples containing different concentrations of c-MET were tested in inde-pendent assays. Acceptable criteria: CV<15%.
Recovery
Recombinant c-MET was spiked into three human serum samples, and then analyzed. The average recovery of c-MET for serum samples is 102.1%.
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Drug Development Progress- English Name：
Hepatocyte growth factor receptor
- Category：
- Approved Drugs：
11 Details
- Drugs in Clinical Trials：
65 Details
- Highest Development Stage：
Phase 2 Clinical
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