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Product Details
Product Overview
Human DLL3 ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human DLL3 that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteDLL3Format96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity25 pg/mLAssay Time2.75 hrSample volume100 μLRange54.69 pg/mL-3500 pg/mLSample TypeFor the quantitative determination of human DLL3 in serum, plasma and cell supernatant.Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant human DLL3 in serum, plasma and cell culture supernatants.
It is for research use only.
Workflow
Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA049-C01Pre-coated Anti-DLL3 Antibody Microplate1 plateCEA049-C02Human DLL3 Standard20 μg×2CEA049-C03Biotin-Anti-DLL3 Antibody Con. Solution100 μLCEA049-C04Biotin-Antibody Dilution Buffer8 mLCEA049-C05Streptavidin-HRP Con. Solution500 μLCEA049-C06Streptavidin-HRP Dilution Buffer15 mLCEA049-C0720×Washing Buffer50 mLCEA049-C08Sample Dilution Buffer15 mL×2CEA049-C09Substrate Solution12 mLCEA049-C10Stop Solution6 mLACRO Quality Management System
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Performance Data
Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was cal-culated based on the results of the standard curve. The minimum detectable concentration of DLL3 is less than 25 pg/mL.
Dilution Linearity
High concentrations of human DLL3 serum samples were diluted with 1:2, 1:4, 1:8, 1:16 ratios for gradient dilution to evaluate the linearity of the assay. In the serum samples, the average detection rate of DLL3 was 96.1%.
Intra-Assay Statistics
Ten replicates of each of four samples containing different DLL3 concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Inter-Assay Statistics
Five samples containing different concentrations of DLL3 were tested in the independent assays. Acceptable criteria: CV<15%.
Recovery
DLL3 was spiked into three human serum samples, and then analyzed. The average re-covery of DLL3 for serum samples is 90.5%.
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Drug Development Progress- English Name：
Delta-like protein 3
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
17 Details
- Highest Development Stage：
Approved
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