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Product Details
Specificity
Specifically recognizes MMAE and does not recognize MMAF.
Source
Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1D12) is a Rabbit monoclonal antibody recombinantly expressed from HEK293 cells.
Clone
M1D12
Isotype
Rabbit IgG | Rabbit Kappa
Conjugate
Unconjugated
Immunogen
MMAE-BSA
Application
ApplicationRecommended UsageELISA0.06-125 ng/mLPurification
Protein A purified / Protein G purified
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
Bioactivity-ELISA
Immobilized Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1D12) (Cat. No. MME-MY2211) at 5 μg/mL, add Disitamab Vedotin (RC48) in the 100% Human Serum and then add Biotinylated Human Her2, His,Avitag, premium grade (Cat. No. HE2-H82E2) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (QC tested).
Protocol
Immobilized Disitamab Vedotin (RC48) at 0.2 μg/mL (100 μL/well) can bind Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1D12) (Cat. No. MME-MY2211) with a linear range of 0.06-2 ng/mL (Routinely tested).
Protocol
Immobilized Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1D12) (Cat. No. MME-MY2211) at 5 μg/mL, add Disitamab Vedotin (RC48) and then add Biotinylated Human Her2, His,Avitag, premium grade (Cat. No. HE2-H82E2) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (Routinely tested).
Protocol
Serial dilutions of Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1D12) (Cat. No. MME-MY2211): Disitamab Vedotin (RC48) binding reactions. The half maximal inhibitory concentration (IC50) is 0.006187 μg/mL (Routinely tested).
Protocol
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