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Mouse IgG1 Kappa Isotype Control (mAb, Carrier free, MALS verified)

COADS MS
DS

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Cat. No. / Size
Price
Qty
DNP-M1-100ug
$165.00
DNP-M1-1mg
$720.00
DNP-M1-25mg
$1545.00
DNP-M1-100mg
$5150.00
ETA of in-stock products:2 business days
Sub-Total$ 0
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Product Details

  • Source

    Mouse IgG1 Kappa Isotype Control (mAb) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.

  • Species

    Mouse

  • Isotype

    Mouse IgG1 | kappa

  • Antibody Type

    Recombinant Monoclonal

  • Specificity

    Specifically reacts with DNP (Dinitrophenyl) and DNP conjugated proteins.

  • Application

    Application
    Recommended Usage
    ELISA
    40-150 ng/mL

  • Purity

    90% as determined by SDS-PAGE.

    95% as determined by SEC-MALS.

  • Purification

    Protein A purified / Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

    25 mg or larger size will be supplied as liquid and shipped by dry ice. Please inquire the dry ice shipping cost.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    IgG control SDS-PAGE

    Mouse IgG1 Kappa Isotype Control (mAb) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • SEC-MALS

    IgG control SEC-MALS

    The purity of Mouse IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-M1) is more than 95% and the molecular weight of this protein is around 130-160 kDa verified by SEC-MALS.

    Report

  • Bioactivity-ELISA

    IgG control ELISA

    Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Mouse IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-M1) with a linear range of 2-20 ng/mL (QC tested).

    Protocol

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Background

A hapten is a small molecule that can elicit an immune response only when conjugated with a large carrier such as a protein. Typical haptens include drugs, urushiol, quinone, steroids, etc. Peptides and non-protein antigens usually need conjugating to a carrier protein (such as BSA (bovine serum albumin) or KLH (keyhole limpet hemocyanin) to become good immunogens). Additionally, haptens should be administered with an adjuvant to ensure a high quality immune response. It is important that the hapten design (preserving greatly the chemical structure and spatial conformation of target compound), selection of the appropriate carrier protein and the conjugation method are key conditions for the desired specificity anti-hapten antibodies. We design anti-hapten antibodies based on the HaptenDB information.

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