Product Details
Source
Monoclonal Anti-HA (A/Wisconsin/588/2019 (H1N1)) Antibody, Mouse IgG1 (6D1) is a Mouse monoclonal antibody produced from a hybridoma created by fusing SP2/0 myeloma and Mouse B-lymphocytes.
Clone
6D1
Species
Mouse
Isotype
Mouse IgG1 | Mouse Kappa
Conjugate
Unconjugated
Antibody Type
Hybridoma Monoclonal
Reactivity
Virus
Immunogen
Recombinant Influenza A [A/Wisconsin/588/2019 (H1N1)] HA derived from human 293 cells
Specificity
This product is a specific antibody specifically reacts with Hemagglutinin/HA (Influenza Virus).
Application
ApplicationRecommended UsageELISA0.1-100 ng/mLPurity
95% as determined by SDS-PAGE.
95% as determined by SEC-MALS.
Purification
Protein A purified / Protein G purified
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Cross Verification
This product can cross in Elisa with
Influenza A [Victoria/4897/2022] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H8).
Influenza A [Wisconsin/67/2022] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H7).
Influenza A [A/Victoria/2570/2019] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H6).
Influenza A [Sydney/5/2021 (H1N1)] HA Protein, His Tag (Cat. No. HA1-V52H14).
No cross-reactivity in ELISA with
Influenza A [A/Darwin/6/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H5).
Influenza A [A/Darwin/9/2021 (H3N2)] HA Protein, His Tag (Cat. No.HA2-V52H6).
Influenza A [A/Hong Kong/483/97 (H5N1)] HA, His Tag (Cat. No. HA1-V5229).
Influenza A (Guangdong/18SF020(H5N6)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA6-V52H3).
Influenza A (turkey/Germany-MV/R2472/2014(H5N8)) HA Protein, His Tag (Cat. No. HA8-V52H3).
Influenza A (A/Shanghai/02/2013(H7N9)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA9-V52H3).
Influenza B [Austria/1359417/2021 (B/Victoria lineage)] Hemagglutinin (HA) Protein, His Tag (Cat. No. HAE-V52H3).
Influenza B [Phuket/3073/2013 (B/Yamagata lineage)] HA Protein, His Tag (Cat. No. HAE-V52H4).
Influenza A (Vietnam/1194/2004(H5N1)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H9).
Influenza A Virus (A/District of Columbia/27/2023) HA (H3N2) Protein, His Tag (Cat. No. H32-V52H5).
Influenza A Virus (A/Croatia/10136RV/2023) HA (H3N2) Protein, His Tag (Cat. No. H32-V52H4).ACRO Quality Management System
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Performance Data
SDS-PAGE

Monoclonal Anti-HA (A/Wisconsin/588/2019 (H1N1)) Antibody, Mouse IgG1 (6D1) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Monoclonal Anti-HA (A/Wisconsin/588/2019 (H1N1)) Antibody, Mouse IgG1 (6D1) (Cat. No. HA1-Y159) is more than 95% and the molecular weight of this protein is around 135-160 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Influenza A [A/Wisconsin/588/2019 (H1N1)] Hemagglutinin (HA) Protein, His Tag (MALS verified) (Cat. No. HA1-V52H3) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-HA (A/Wisconsin/588/2019 (H1N1)) Antibody, Mouse IgG1 (6D1) (Cat. No. HA1-Y159) with a linear range of 0.049-3.125 ng/mL. (QC tested).
Protocol
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