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Monoclonal Anti-gC-HSV-2 (strain 333) Antibody, Mouse IgG1 (3A12) (MALS verified)

COADS MS
DS

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Cat. No. / Size
Price
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HSV-Y184-100ug
$415.00
HSV-Y184-1mg
$2735.00
ETA of in-stock products:2 business days
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Product Details

  • Source

    Monoclonal Anti-gC-HSV-2 (strain 333) Antibody, Mouse IgG1 (3A12) is a Mouse monoclonal antibody produced from a hybridoma created by fusing SP2/0 myeloma and Mouse B-lymphocytes.

  • Clone

    3A12

  • Species

    Mouse

  • Isotype

    Mouse IgG1 | Mouse Kappa

  • Conjugate

    Unconjugated

  • Antibody Type

    Hybridoma Monoclonal

  • Reactivity

    Virus

  • Immunogen

    Recombinant HSV-2 (strain 333) Envelope Glycoprotein C (gC) derived from human 293 cells

  • Specificity

    This product is a specific antibody specifically reacts with Glycoprotein C/gC (HSV).

  • Application

    Application
    Recommended Usage
    ELISA
    0.2-100 ng/mL

  • Purity

    95% as determined by SDS-PAGE.

    90% as determined by SEC-MALS.

  • Purification

    Protein A purified / Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Glycoprotein C/gC (HSV) SDS-PAGE

    Monoclonal Anti-gC-HSV-2 (strain 333) Antibody, Mouse IgG1 (3A12) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Glycoprotein C/gC (HSV) SEC-MALS

    The purity of Monoclonal Anti-gC-HSV-2 (strain 333) Antibody, Mouse IgG1 (3A12) (Cat. No. HSV-Y184) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.

    Report

  • Bioactivity-ELISA

    Glycoprotein C/gC (HSV) ELISA

    Immobilized HSV-2 (strain 333) Envelope Glycoprotein C (gC), His Tag (MALS verified) (Cat. No. GLC-V52H3) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-gC-HSV-2 (strain 333) Antibody, Mouse IgG1 (3A12) (Cat. No. HSV-Y184) with a linear range of 0.098-6.25 ng/mL (QC tested).

    Protocol

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Background

Herpesvirus infections are widely spread throughout the world population. Herpes simplex virus (HSV) belongs to the α-herpesvirus subfamily. There are two main types of HSV, HSV-1 and HSV-2, which infect humans. HSV-2 mainly causes genital lesions, whereas HSV-1 is involved in both oral and genital infections. Glycoprotein C (gC) is a structural component of the herpes simplex virus type 2 (HSV-2) envelope that mediates binding of the virus to cell surface heparan sulfate or chondroitin sulfate. Also plays a role in host immune evasion by inhibiting the host complement cascade activation (By similarity).

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