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VEGF: Cutting off tumor's feeding channel

Not only vascular endothelial growth factor (VEGF) plays a role in promoting angiogenesis and regeneration, but shows multiple functions such as immune regulation and neuroprotection. VEGF family members exert their effects through interactions with a family of three VEGF receptors. VEGF is up-regulated when it overexpressed in many tumors. Therefore, it becomes a hot target of drug development for tumors.
Currently, there are more than 100 antibody drugs, such as monoclonal antibodies (mAb) and bispecific antibodies (BsAb) against VEGF are widely-used for clinical treatment.
Given its significant role in multiple cancer types, including tumors, intraocular neovascular disorders and other cardiovascular diseases, there is no surprise that VEGF has been heavily studied for new target therapeutics.
ACROBiosystems has developed a variety of VEGF proteins with different molecules, subtypes, species, and tags to help expedite your antibody drug development. All SPR&BLI protocols are available for free.

Product list

VEGF-A
VEGF-B
VEGF-C
VEGF-D
VEGF R1
VEGF R2
VEGF R3

Product Features

High purity verified by MALS
High purity verified by MALS
The purity of ActiveMax® Human VEGF165, Tag Free (MALS verified) (Cat. No. VE5-H4210) is more than 95% in HP-SEC, and the molecular weight of this protein is around 40-55 kDa verified by SEC-MALS.
High bioactivity validated by ELISA&SPR&BLI&cell based assay
>>ELISA
High purity verified by MALS

Immobilized Human VEGF R1&VEGF R2, Fc Tag at 2 μg/mL (100 μL/well) can bind Human VEGF165, His Tag (Cat. No. VE5-H5248) with a linear range of 0.004-0.063 μg/mL (QC tested).

Protocol

High purity verified by MALS

Immobilized Human VEGF165, premium grade (MALS verified) (Cat. No. VE5-H4210) at 0.1 μg/mL (100 μL/well) can bind Human VEGF R1 Protein, His Tag (Cat. No. VE1-H52H9) with a linear range of 1.2-10 ng/mL (QC tested).

Protocol

>>SPR
High purity verified by MALS

Immobilized Biotinylated Human VEGF165, His,Avitag (Cat. No. VE5-H82Q0) on CM5 Chip via Streptavidin, can bind Avastin with an affinity constant of 0.417 nM as determined in SPR assay (Biacore T200) (Routinely tested).

Protocol

>>BLI
High purity verified by MALS

Loaded Avastin (Bevacizumab) on AHC Biosensor, can bind Human VEGF165, His Tag (Cat. No. VE5-H5248) with an affinity constant of 0.448 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

>>Cell based assay
High purity verified by MALS

ActiveMax® Human VEGF165, Tag Free (MALS verified) (Cat. No. VE5-H4210) stimulates proliferation of human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is 4.216-9.281 ng/mL (Routinely tested).

Protocol

High purity verified by MALS

Inhibition assay shows that the proliferation effect of ActiveMax® Human VEGF165, Tag Free (MALS verified) (Cat. No. VE5-H4210) is inhibited by increasing concentration of anti-VEGF mAb (Avastin). The concentration of VEGF165 used is 20 ng/mL. The ED50 is 0.065-0.229 μg/mL (Routinely tested).

Protocol

Citations

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