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Your Position: Home > Protein > Erythropoietin R > EPR-H52H4

Human Erythropoietin R / EPO R Protein, His Tag (MALS & SPR verified)

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  • Synonym
    EPOR
  • Source
    Human Erythropoietin R Protein, His Tag(EPR-H52H4) is expressed from human 293 cells (HEK293). It contains AA Ala 25 - Pro 250 (Accession # P19235-1).
    Predicted N-terminus: Ala 25
  • Molecular Characterization
    Erythropoietin R Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 26.7 kDa. The protein migrates as 30-32 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Erythropoietin R SDS-PAGE

Human Erythropoietin R Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
Erythropoietin R MALS images

The purity of Human Erythropoietin R Protein, His Tag (Cat. No. EPR-H52H4) is more than 85% and the molecular weight of this protein is around 25-38 kDa verified by SEC-MALS.

Bioactivity-SPR
 Erythropoietin R SPR

Human Erythropoietin R Protein, His Tag (Cat. No. EPR-H52H4) immobilized on CM5 Chip can bind Human EPO Protein, premium grade (Cat. No. EPO-H4214) with an affinity constant of 0.142 nM as determined in a SPR assay (Biacore 8K) (QC tested).

Bioactivity-Bioactivity CELL BASE
 Erythropoietin R CELL

Human Erythropoietin R Protein, His Tag (Cat. No. EPR-H52H4) inhibits human erythropoietin/EPO-dependent proliferation of TF-1 cells. The EC50 is 211.1 ng/mL (Routinely tested).

  • Background
    This gene encodes the erythropoietin receptor which is a member of the cytokine receptor family. Upon erythropoietin binding, this receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphat.
  • Clinical and Translational Updates

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