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This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 31 kDa. The protein migrates as 40-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human VEGF-C Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
The purity of Human VEGF-C Protein, His Tag (Cat. No. VEC-H52H3) is more than 90% and the molecular weight of this protein is around 30-45 kDa verified by SEC-MALS.
Immobilized Human VEGF R3, Fc Tag (Cat. No. FL4-H5251) at 5 μg/mL (100 μL/well) can bind Human VEGF-C Protein, His Tag (Cat. No. VEC-H52H3) with a linear range of 1-16 ng/mL (QC tested).
Human VEGF-C Protein, His Tag (Cat. No. VEC-H52H3) capture on NTA-Series S sensor chip can bind Human VEGF R3, Fc Tag (Cat. No. FL4-H5251) with an affinity constant of 0.130 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
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