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This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 55.7 kDa. The protein migrates as 60-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Hendra virus Pre-Fusion glycoprotein (A263T), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Immobilized Anti-Fusion Protein Antibody, Human IgG1 (5B3) at 1 μg/mL (100 μL/well) can bind Hendra virus Pre-Fusion glycoprotein (A263T), His Tag (Cat. No. FUN-H52H4) with a linear range of 1-2 ng/mL (QC tested).
Loaded Anti-Fusion Protein Antibody, Human IgG1 (5B3) on AHC Biosensor, can bind Hendra virus Pre-Fusion glycoprotein (A263T), His Tag (Cat. No. FUN-H52H4) with an affinity constant of 10.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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