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Your Position: Home > Antibody > Cetuximab > CEB-Y28

Anti-Cetuximab Antibody (AY28)

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  • Source

    Anti-Cetuximab Antibody (AY28) is a Mouse monoclonal antibody produced from a hybridoma created by fusing SP2/0 myeloma and Mouse B-lymphocytes.

  • Clone

    AY28

  • Species

    Mouse

  • Isotype

    Mouse IgG1/kappa

  • Antibody Type

    Hybridoma Monoclonal

  • Reactivity

    Human

  • Immunogen

    Cetuximab.

  • Specificity

    Recognizes Cetuximab specifically, no cross reactivity with other humanized antibodies.

  • Application
    ApplicationRecommended Usage
    ELISA0.2-25 ng/mL
  • Purity

    >95% as determined by SDS-PAGE.

  • Purification

    Protein A purified/ Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
SDS-PAGE
Cetuximab SDS-PAGE

Anti-Cetuximab Antibody (AY28) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA
 Cetuximab ELISA

Immobilized Cetuximab (Cat. No. cetuximab) at 1 μg/mL (100 μL/well) can bind Anti-Cetuximab Antibodies (Cat. No. CEB-Y28) with a linear range of 0.2-3 ng/mL (QC tested).

 Cetuximab ELISA

Anti-Cetuximab Antibodies bridging ELISA for Anti-Drug Antibody (ADA) assay development. Immobilized cetuximab at 1 µg/mL, add increasing concentrations of Anti-Cetuximab Antibody (AY28) (Cat. No. CEB-Y28, 10% human serum) and then add biotinylated cetuximab at 5 µg/mL. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 2.4 ng/mL.

 Cetuximab ELISA

Demonstration of the specificity of Anti-Cetuximab Antibody (AY28) (Cat. No. CEB-Y28) to the cetuximab.

 Cetuximab ELISA

Measured by its neutralizing ability in a functional ELISA. Immobilized cetuximab at 0.2 μg/mL (100 μL/well) can bind pre-mixed Anti-Cetuximab Antibody (AY28) (Cat. No. CEB-Y28) and Biotinylated Human EGFRvIII, His,Avitag (Cat. No. EGR-H82E0) with an inhibition rate of 100%.

Bioactivity-SPR
 Cetuximab SPR

Anti-Cetuximab Antibody (AY28) (mouse IgG1, Cat. No. CEB-Y28) captured on CM5 chip via anti-mouse antibodies surface, can bind Human cetuximab with an affinity constant of 1.51 pM.

  • Background
    Cetuximab is an epidermal growth factor receptor binding FAB. Cetuximab is composed of the Fv (variable; antigen-binding) regions of the 225 murine EGFr monoclonal antibody specific for the N-terminal portion of human EGFr with human IgG1 heavy and kappa light chain constant (framework) regions.
  • Clinical and Translational Updates

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