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Your Position: Home > Pseudovirus Neutralization Antibody Assay Service

Pseudovirus Neutralization Assay Service

Background

SARS-COV-2 Spike Pseudovirus: A kind of chimeric virus particles formed by replacing the envelope protein in the HIV-1 virus skeleton with SARS-COV-2 Spike. Pseudovirus can simulate the process of SARS-CoV-2 infecting cells. The nucleic acid in the virus particle is artificially designed and modified to make the virus replication-defective and not pathogenic.

As SARS-CoV-2 is highly infectious and pathogenic, so it considerably increases the research difficulty and safety hazards for researchers. Pseudoviruses have the essential components for cell entry and viral infection, but lack of nucleic acid and lose self-replication ability. They have been regarded as the effective virological tools due to their safety and versatility. It is currently widely used in the screening and evaluation of antibodies and antiviral drugs, neutralizing antibody assay, and vaccine efficacy evaluation.As a leading company, ACROBiosystems is committed to accelerating the development of reagent products in the drug research and development field , we also hope to accelerate the development of anti-epidemic drugs through our company's rich technical service experience. ACROBiosystems is now launching the SARS-COV-2 Spike Pseudovirus research-related services and products to help the development of antiviral drugs!

Platform
Principle


Pseudovirus can simulate SARS-CoV-2 to infect target cells


Neutralizer can block pseudovirus from infecting target cells

The SARS-COV-2 Spike Pseudovirus containing the firefly luciferase gene infects target cells and expresses the luciferase gene through its envelope protein—S protein. The S protein is a key component present on the surface of SARS-CoV-2 and is responsible for the viral tropism through host cell recognition by binding to the human angiotensin-converting enzyme 2 (hACE2) receptor. It is the main target for neutralizing antibodies and vaccine design. Neutralizing antibody can block the pseudovirus from infecting host cells. By detecting the decrease in the expression of the luciferase reporter gene, the degree to which the virus is blocked can be inferred to screen or verify the neutralizer.

Process
Service details
Sample typeNeutralizing antibodies, drugs, serum and so on, customers only need to provide samples
Detection reagentProvide the human ACE2 overexpressing cells, SARS-CoV-2 Spike pseudovirus (wild type and mutant), fluorescence detection reagents, positive antibody control, and other materials needed for the experiment
Detection equipmentPerkinElmer EnSight Microplate Reader
Quality StandardThe ratio of the average fluorescence of the pseudovirus to the average fluorescence of the cell control is larger than 1000, and the background value of the fluorescence of the pseudovirus is less than 1%
DeliverableProject report
Service period2-4 weeks

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Case study

Use wild-type (Figures A and B) and Omicron (Figures C and D) S protein pseudovirus to analyze the neutralizing ability of three neutralizing antibodies (SPD-M265, S1N-M122, REGN10987) against SARS-COV-2

SARS-COV-2 Spike Pseudovirus research related products
SARS-CoV-2 Spike (Omicron) Fluc-GFP Pseudovirus• Contains firefly luciferase reporter gene
• The full-length S protein of the SARS-CoV-2 (Omicron) mutant is an envelope protein.
SARS-CoV-2 Spike (Delta) Fluc-GFP Pseudovirus• Contains firefly luciferase reporter gene
• The full-length S protein of the SARS-CoV-2 (Delta) mutant is an envelope protein.
SARS-CoV-2 Spike (WT) Fluc-GFP Pseudovirus• Contains firefly luciferase reporter gene
• The full-length S protein of SARS-CoV-2  is an envelope protein.
SARS-CoV-2 (Omicron) Pseudovirus Neutralization Reagents• SARS-CoV-2 (Omicron) Spike Pseudovirus
• Human ACE2 overexpression cell line
• Validated as an ideal positive control antibody for pseudovirus neutralization experiments
SARS-CoV-2 (Delta) Pseudovirus Neutralization Reagents• SARS-CoV-2 (Delta) Spike Pseudovirus
• Human ACE2 overexpression cell line
• Validated as an ideal positive control antibody for pseudovirus neutralization experiments
SARS-CoV-2 (WT) Pseudovirus Neutralization Reagents• SARS-CoV-2 (WT) Spike Pseudovirus
• Human ACE2 overexpression cell line
• Validated as an ideal positive control antibody for pseudovirus neutralization experiments
Advantage

High stability: The method has high stability and the results are true and reliable.

High specificity and sensitivity: High-quality pseudovirus, high-sensitivity detection equipment recognized by the industry.

High-quality detection model: Highly simulating the physiological infection mechanism, which can simulate the actual treatment effect to the greatest extent.

Efficient and fast :The project team will follow up the project one-on-one, give you feedback on the progress of the project periodically, and deliver the experimental results in time.

Extensive service experience and excellent attitude : Outstanding technical service experience, and customized personalized experimental strategies in close linkage with customers.

Contact us

Contact us: Welcome to send an email to services@acrobiosystems.com for consultation, thank you!

Service flow

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