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Human VEGF R2 / KDR Protein (HPLC-verified)  pdf  pdf  pdf


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Synonym

KDR,CD309,FLK1,VEGFR,VEGFR2

Source

Human VEGF R2, His Tag (HPLC-verified) (KDR-H5227) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Glu 764 (Accession # AAI31823).

Predicted N-terminus: Ala 20

Molecular Characterization

VEGF R2(Ala 20 - Glu 764)AAI31823
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 86.2 kDa. The protein migrates as 100-110 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

>90% as determined by SEC-HPLC.

Bioactivity

Measured by its ability to bind ActiveMax® Human VEGF165 (Cat# VE5-H4210) in the SPR assay (Biacore 2000) with an estimated KD < 50nM.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human VEGF R2, His Tag (HPLC-verified) (Catalog # KDR-H5227) SDS-PAGE gel

Human VEGF R2, His Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC

Human VEGF R2, His Tag (HPLC-verified) (Catalog # KDR-H5227) HPLC images

The purity of Human VEGF R2 (HPLC-verified), His Tag(Cat.# KDR-H5227) was greater than 95% as determined by SEC-HPLC.

 

Background

Kinase insert domain receptor (KDR) is also known as CD309, FLK1, VEGFR, VEGFR2, and is one of the subtypes of VEGFR. VEGF receptors are receptors for vascular endothelial growth factor (VEGF). There are three main subtypes of VEGFR, numbered 1, 2 and 3. The VEGF receptors have an extracellular portion consisting of 7 immunoglobulin-like domains, a single transmembrane spanning region and an intracellular portion containing a split tyrosine-kinase domain. VEGF-A binds to VEGFR-1 (Flt-1) and VEGFR-2 (KDR/Flk-1). VEGFR-2 appears to mediate almost all of the known cellular responses to VEGF.The function of VEGFR-1 is less well defined, although it is thought to modulate VEGFR-2 signaling. Another function of VEGFR-1 may be to act as a dummy/decoy receptor, sequestering VEGF from VEGFR-2 binding (this appears to be particularly important during vasculogenesis in the embryo). In addition, VEGFR2 is able to interact with HIV-1 extracellular Tat protein upon VEGF activation, and seems to enhance angiogenesis in Kaposi's sarcoma lesions.

References

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