Browse Products By Molecule:

Products --> Bio-Markers & CD Antigens

Human CD3 epsilon Protein, His Tag & Fc Tag  pdf  pdf  pdf


Cat-Pack Size Price(USD) Qty Inventory Order
Quote for bulk   Worldwide Shipping & Payment
CDE-H5256-100ug
    $290.00
CDE-H5256-1mg
    $1870.00
In-Stock
Place Purchase Orders


Synonym

FLJ18683, T3E, TCRE, CD3E, CD3-epsilon

Source

Recombinant Human CD3E /CD3 Epsilon Protein, With C-Fc Tag (rh CD3E Fc Chimera) Asp 23 - Asp 126 (Accession # NP_000724.1) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

rh CD3E Fc Chimera is fused with polyhistidine tagged Fc-tag at the C-terminus, and has a calculated MW of 39.2 kDa. The predicted N-terminus is Asp 23. DTT-reduced Protein migrates as 44-54 kDa in SDS-PAGE due to glycosylation.

Endotoxin

Less than 1.0 EU per μg of the rh CD3E Fc Chimera by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Contact us for customized product format or formulation.

Reconstitution

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Recombinant Human CD3E /CD3 Epsilon Protein, With C-Fc Tag
The purity of rh CD3E Fc Chimera was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


References