FLJ18683, T3E, TCRE, CD3E, CD3-epsilon
Recombinant Human CD3E /CD3 Epsilon Protein, With C-Fc Tag (rh CD3E Fc Chimera) Asp 23 - Asp 126 (Accession # NP_000724.1) was produced in human 293 cells (HEK293) at ACROBiosystems.
rh CD3E Fc Chimera is fused with polyhistidine tagged Fc-tag at the C-terminus, and has a calculated MW of 39.2 kDa. The predicted N-terminus is Asp 23. DTT-reduced Protein migrates as 44-54 kDa in SDS-PAGE due to glycosylation.
Less than 1.0 EU per μg of the rh CD3E Fc Chimera by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
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See Certificate of Analysis for details of reconstitution instruction and specific concentration.
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.
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