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Human Mature PCSK9 (153-692) Protein  pdf  pdf  pdf


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Synonym

PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9

Source

Human Mature PCSK9 (153-692) Protein (Human Mature PCSK9 (153-692)) Ser 153 - Gln 692 (Accession # AAI66619) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

Human Mature PCSK9 (153-692) is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 58 kDa. The predicted N-terminus is Ser 153. The reducing (R) protein migrates as 62 kDa in SDS-PAGE due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>97% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 10 mM HCl, pH2.4. Normally Trehalose is added as protectant before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

 

SDS-PAGE

Human Mature PCSK9 (153-692) Protein

Human Mature PCSK9 (153-692) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity

Immobilized Human LDL R, His Tag (Cat# LDR-H5224) at 10 μg/mL (100 μl/well) can bind Human Mature PCSK9 (153-692) (Cat# PC9-H5226) with a linear range of 0.03-0.5 μg/mL.

 
 

Background

Proprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene.This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).

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References