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Human Her2 / ErbB2 Protein, Strep Tag  pdf  pdf  pdf


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HE2-H5287-50ug
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Synonym

ERBB2,CD340,HER-2/neu,HER2,MLN19,NEU,NGL,TKR1

Source

Human Her2 / ErbB2 Protein, Strep Tag (Human Her2, Strep Tag) Thr 23 - Thr 652 (Accession # AAA75493) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

Her2 (Thr 23-Thr 652)AAA75493

Twin Strep

Human Her2, Strep Tag is fused with twin strep tag at the C-terminus, and has a calculated MW of 72.4 kDa. The predicted N-terminus is Thr 23. The reducing (R) protein migrates as 110-115 kDa in SDS-PAGE due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

 

SDS-PAGE

Human Her2 / ErbB2 Protein, Strep Tag

Human Her2, Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Immobilized Human Her2, Strep Tag (Cat# HE2-H5287) at 2 μg/mL (100 μl/well) can bind trastuzumab with a linear range of 0.1-2 ng/mL.

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Background

Human Epidermal growth factor Receptor 2 (HER2) is also called ERBB2, HER-2,HER-2 /neu, NEU, NGL,TKR1 and c-erb B2,and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. Approximately 30% of breast cancers have an amplification of the HER2 gene or overexpression of its protein product. Overexpression of this receptor in breast cancer is associated with increased disease recurrence and worse prognosis. HER2 appears to play roles in development, cancer,communication at the neuromuscular junction andregulation of cell growth and differentiation.

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References