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Human PD-L1 / B7-H1 Protein, Strep Tag  pdf  pdf  pdf


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PD1-H5282-25ug
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Synonym

PD-L1,CD274,B7-H1,PDCD1L1,PDCD1LG1

Source

Human PD-L1 / B7-H1 Protein, Strep Tag, Phe 19 - Arg 238 (Accession # NP_054862.1) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

PD-L1 (Phe 19-Arg 238)NP_054862.1

Twin-Strep

Human PD-L1, Strep Tag is fused with twin strep tag at the C-terminus, and has a calculated MW of 28.2 kDa. The predicted N-terminus is Phe 19. The reducing (R) protein migrates as 35-42 kDa in SDS-PAGE due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

 

SDS-PAGE

Human PD-L1, Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Immobilized Human PD-L1, Strep Tag (Catalog # PD1-H5282) at 5 μg/mL ( 100 μl/well ) can bind Biotinylated Human PD-1, His Tag & Fc tag (Catalog # PD1-H82F2) with a linear range of 2-60 ng/mL.

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Background

Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.

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References