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Human PD-L1 / B7-H1 Protein, Strep Tag  pdf  pdf  pdf


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Synonym

PD-L1,CD274,B7-H1,PDCD1L1,PDCD1LG1

Source

Human PD-L1, Strep Tag (PD1-H5282) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1).

Predicted N-terminus: Phe 19

Molecular Characterization

PD-L1(Phe 19 - Arg 238)NP_054862.1
Twin-Strep

This protein carries a twin strep tag at the C-terminus.

The protein has a calculated MW of 28.2 kDa. The protein migrates as 35-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human PD-L1, Strep Tag (Cat. No. PD1-H5282) SDS-PAGE gel

Human PD-L1, Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Human PD-L1, Strep Tag (Cat. No. PD1-H5282) ELISA bioactivity

Immobilized Human PD-L1, Strep Tag (Cat. No. PD1-H5282) at 5 μg/mL ( 100 μl/well ) can bind Biotinylated Human PD-1, His Tag & Fc tag (Cat. No. PD1-H82F2) with a linear range of 2-60 ng/mL (QC tested).

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Background

Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.

References

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