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| Assay Type | Sandwich-ELISA |
| Analyte | bFGF/FGF2 |
| Format | 96T |
| Reactivity | Human |
| Sensitivity | 7 pg/ml |
| Standard Curve Range | 10.94 pg/mL-700 pg/mL |
| Assay Time | 2.75 hr |
| Suitable Sample Type | For the quantitative determination of human bFGF/FGF2 in Cell Culture Supernatants, Plasma, Serum. |
| Sample volume | 50 uL |
| ID | Components | Size |
| CEA082-C01 | Pre-coated Anti-bFGF/FGF2 Antibody Microplate | 1 plate |
| CEA082-C02 | Human bFGF/FGF2 Standard | 20μg ×2 |
| CEA082-C03 | Biotin-Anti-bFGF/FGF2 Antibody Con. Solution | 100 μL |
| CEA082-C04 | Biotin-Antibody Dilution Buffer | 8 mL |
| CEA082-C05 | Streptavidin-HRP Con. Solution | 500 uL |
| CEA082-C06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CEA082-C07 | 20× Washing Buffer | 50 mL |
| CEA082-C08 | Sample Dilution Buffer | 15 mL ×2 |
| CEA082-C09 | Substrate Solution | 12 mL |
| CEA082-C10 | Stop Solution | 6 mL |
A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.
ClinMax™ ELISA Kits are manufactured in a GMP-like facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.
The kit is developed for quantitative detection of human bFGF/FGF2 in serum, plasma and cell culture supernates.
It is for research use only.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of bFGF/FGF2 is less than 150 pg/mL.
Ten replicates of each of 3 samples containing different bFGF/FGF2 concentrations were tested in one assay. Acceptable criteria: CV＜10%.
3 samples containing different concentrations of bFGF/FGF2 were tested in independent assays. Acceptable criteria: CV<15%.
Recombinant bFGF/FGF2 was spiked into 3 human serum samples, and then analyzed. The average recovery of bFGF/FGF2 for serum samples is 100.9%.
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