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Human Her2 / ErbB2 Protein, C13 and N15-labeled  pdf  pdf  pdf


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HE2-H9225-5ug
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Synonym

ERBB2, CD340, HER-2/neu, HER2, MLN19, NEU, NGL, TKR1

Source

Recombinant Human HER2 /ErbB2 MS Standard Protein, C13 and N15-labeled (HER2 / ErbB2, Heavy Labeled) Thr 23 - Thr 652 (Accession # AAA75493) was produced in human 293 cells (HEK293) with fully chemically defined cell culture medium to obtain >99% incorporation efficiency at ACROBiosystems.

Molecular Characterization

rh HER2 / ErbB2, Heavy Labeled is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 70.2 kDa. The predicted N-terminus is Thr 23. DTT-reduced Protein migrates as 110-115 kDa in SDS-PAGE due to glycosylation.

HER2 / ErbB2, Heavy Labeled is labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine

Endotoxin

Less than 1.0 EU per μg of the rh HER2 / ErbB2, Heavy Labeled by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Recombinant Human HER2 /ErbB2 MS Standard Protein, C13 and N15-labeled
The purity of rh HER2 / ErbB2, Heavy Labeled was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

Human Epidermal growth factor Receptor 2 (HER2) is also called ERBB2, HER-2,HER-2 /neu, NEU, NGL,TKR1 and c-erb B2,and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. Approximately 30% of breast cancers have an amplification of the HER2 gene or overexpression of its protein product. Overexpression of this receptor in breast cancer is associated with increased disease recurrence and worse prognosis. HER2 appears to play roles in development, cancer,communication at the neuromuscular junction andregulation of cell growth and differentiation.

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References