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Human EGF R Protein, C13 and N15-labeled  pdf  pdf  pdf


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EGR-H9222-5ug
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EGR-H9222-20ug
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Synonym

EGFR,ERBB,ERBB1,HER1,PIG61,mENA

Source

Recombinant Human EGFR MS Standard Protein, C13 and N15-labeled (EGFR / ErbB1, Heavy Labeled) Leu 25 - Ser 645 (Accession # NP_005219) was produced in human 293 cells (HEK293) with fully chemically defined cell culture medium to obtain >99% incorporation efficiency at ACROBiosystems.

Predicted N-terminus: Leu 25

Molecular Characterization

EGF R(Leu 25 - Ser 645)NP_005219
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 69.5 kDa. The protein migrates as 110-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

EGFR / ErbB1, Heavy Labeled is labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

C13 and N15-labeled, Human EGF R (Cat. No. EGR-H9222) SDS-PAGE gel

C13 and N15-labeled, Human EGF R on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Background

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


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