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Human EGF R Protein, C13 and N15-labeled  pdf  pdf  pdf


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EGR-H9222-20ug
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Synonym

EGFR, ERBB, ERBB1, HER1, PIG61, mENA

Source

Recombinant Human EGFR MS Standard Protein, C13 and N15-labeled (EGFR / ErbB1, Heavy Labeled) Leu 25 - Ser 645 (Accession # NP_005219) was produced in human 293 cells (HEK293) with fully chemically defined cell culture medium to obtain >99% incorporation efficiency at ACROBiosystems.

Molecular Characterization

EGFR / ErbB1, Heavy Labeled is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 69.5 kDa. The predicted N-terminus is Leu 25. DTT-reduced Protein migrates as 110-115 kDa in SDS-PAGE due to glycosylation.

EGFR / ErbB1, Heavy Labeled is labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine

Endotoxin

Less than 1.0 EU per μg of the EGFR / ErbB1, Heavy Labeled by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Recombinant Human EGFR MS Standard Protein, C13 and N15-labeled
The purity of EGFR / ErbB1, Heavy Labeled was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


References