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Your Position: Home > Protein > Fc gamma RIIB/C / CD32b/c > CDB-H5228

Human Fc gamma RIIB/C (CD32b/c) Protein, His Tag (SPR & BLI & MALS verified)

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  • Synonym
    FCGR2B,C,CD32b,c,FcRII-b,c,Fc-gamma RII-b,c,Fc-gamma-RIIb,c,CD32,FCG2,IGFR2,CDw32
  • Source
    Human CD32b/c, His Tag (CDB-H5228) is expressed from human 293 cells (HEK293). It contains AA Ala 46 - Pro 217 (Accession # P31994-1). In the region Ala 46 - Pro 217, the AA sequence of Fc gamma RIIB and Fc gamma RIIC are homologus.
    Predicted N-terminus: Ala 46
  • Molecular Characterization
    Fc gamma RIIB/C / CD32b/c Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 20.4 kDa. The protein migrates as 28-31 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to different glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
SDS-PAGE
Fc gamma RIIB/C / CD32b/c SDS-PAGE

Human CD32b/c, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
Fc gamma RIIB/C / CD32b/c MALS images

The purity of Human CD32b/c, His Tag (Cat. No. CDB-H5228) is more than 90% and the molecular weight of this protein is around 20-30 kDa verified by SEC-MALS.

Bioactivity-SPR
 Fc gamma RIIB/C / CD32b/c SPR

Immobilized Human CD32b/c, His Tag (Cat. No. CDB-H5228) on CM5 Chip via anti-His antibody, can bind Rituximab with an affinity constant of 15.1 μM as determined in a SPR assay (Biacore T200) (QC tested).

 Fc gamma RIIB/C / CD32b/c SPR

Immobilized Rituximab on CM5 Chip, can bind Human CD32b/c, His Tag (Cat. No. CDB-H5228) with an affinity constant of 5.5 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

 Fc gamma RIIB/C / CD32b/c SPR

Rituximab captured on Protein A Chip can bind Human CD32b/c, His Tag (Cat. No. CDB-H5228) with an affinity constant of 3.56 μM as determined in SPR assay (Biacore 8K) (Routinely tested).

 Fc gamma RIIB/C / CD32b/c SPR

Rituximab immobilized on CM5 Chip can bind Human CD32b/c, His Tag (Cat. No. CDB-H5228) with an affinity constant of 4.09 μM as determined in SPR assay (Biacore 8K) (Routinely tested).

Bioactivity-BLI
 Fc gamma RIIB/C / CD32b/c BLI

Loaded Human CD32b/c, His Tag (Cat. No. CDB-H5228) on HIS1K Biosensor, can bind Rituximab with an affinity constant of 4.30 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

 Fc gamma RIIB/C / CD32b/c BLI

Loaded Rituximab on FAB2G Biosensor, can bind Human CD32b/c, His Tag (Cat. No. CDB-H5228) with an affinity constant of 5.4 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized.
    There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. Low affinity immunoglobulin gamma Fc region receptor II-b (FCGR2B) is also known as CD32b, FCG2, IGFR2. CD32B is expressed on B cells and myeloid dendritic cells. Ligation of CD32B on B cells downregulates antibody production and may, in some circumstances, promote apoptosis. Co-ligation of CD32B on dendritic cells inhibits maturation and blocks cell activation. CD32B may also be a target for monoclonal antibody therapy for malignancies.
  • Clinical and Translational Updates

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