Human VEGF R1, His Tag (VE1-H5220) is expressed from human 293 cells (HEK293). It contains AA Ser 27 - Asn 756 (Accession # NP_002010.1).
Predicted N-terminus: Ser 27
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 83 kDa. The protein migrates as 115-125 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>98% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
- 4-8°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human VEGF R1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.
(1)Immobilized ActiveMax® Human VEGF165 (Catalog # VE5-H4210) at 2 μg/mL can bind Human VEGF R1, His Tag (Catalog # VE1-H5220) with a linear range of 3-50 ng/mL.
(2)Measured by its ability to bind ActiveMax® Human VEGF165 (Cat# VE5-H4210) in the SPR assay (Biacore 2000) with an estimated KD of 0.1nM (routinely tested).
Vascular endothelial growth factor receptor 1 (VEGFR1) is also known as Fms-like tyrosine kinase 1 (FLT-1), Tyrosine-protein kinase receptor FLT, is a single-pass type I membrane protein and secreted protein which belongs to the protein kinase superfamily, Tyr protein kinase family and CSF-1/PDGF receptor subfamily. VEGFR1 is detected in normal lung, but also in placenta, liver, kidney, heart and brain tissues and specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. VEGFR1 acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. VEGFR1 may play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. VEGFR1 can promote endothelial cell proliferation, survival and angiogenesis in adulthood.
- (1) Shibuya M., et al., 1990, Oncogene 5:519-524.
- (2) Kendall R.L., et al., 1993, Proc. Natl. Acad. Sci. U.S.A. 90:10705-10709.
- (3) Seetharam L., et al., 1995, Oncogene 10:135-147.
- (4) Barleon B., et al., 1996, Blood 87:3336-3343.
- (5) Grunewald F.S., et al., 2010, Biochim. Biophys. Acta 1804:567-580.
- (6) Iyer S., et al., 2010, J. Biol. Chem. 285:23779-23789.
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