Browse Products By Molecule:

This web search service is supported by Google Inc.

Products --> Bio-Markers & CD Antigens

Human VEGF R1 / Flt-1 Protein  pdf  pdf  pdf


Cat-Pack Size Price(USD) Qty Inventory Order
Quote for bulk   Worldwide Shipping & Payment
VE1-H5220-100ug
    $290.00
In-stock
Place Purchase Orders


Synonym

FLT,VEGFR1,FLT1

Source

Human VEGF R1, His Tag (VE1-H5220) is expressed from human 293 cells (HEK293). It contains AA Ser 27 - Asn 756 (Accession # NP_002010.1).

Predicted N-terminus: Ser 27

Molecular Characterization

VEGF R1(Ser 27 - Asn 756)NP_002010.1
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 83 kDa. The protein migrates as 115-125 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>98% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human VEGF R1, His Tag (Cat. No. VE1-H5220) SDS-PAGE gel

Human VEGF R1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.

Bioactivity

Human VEGF R1, His Tag (Cat. No. VE1-H5220) ELISA bioactivity

(1) Immobilized ActiveMax® Human VEGF165 (Cat. No. VE5-H4210) at 2 μg/mL can bind Human VEGF R1, His Tag (Cat. No. VE1-H5220) with a linear range of 4-31 ng/mL (QC tested).

Request for protocol

(2) Measured by its ability to bind ActiveMax® Human VEGF165 (Cat. No. VE5-H4210) in the SPR assay (Biacore 2000) with an estimated KD of 0.1nM (Routinely tested).

 

Background

Vascular endothelial growth factor receptor 1 (VEGFR1) is also known as Fms-like tyrosine kinase 1 (FLT-1), Tyrosine-protein kinase receptor FLT, is a single-pass type I membrane protein and secreted protein which belongs to the protein kinase superfamily, Tyr protein kinase family and CSF-1/PDGF receptor subfamily. VEGFR1 is detected in normal lung, but also in placenta, liver, kidney, heart and brain tissues and specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. VEGFR1 acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. VEGFR1 may play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. VEGFR1 can promote endothelial cell proliferation, survival and angiogenesis in adulthood.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.