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Mouse PCSK9 Protein  pdf  pdf  pdf


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PC9-M5228-100ug
    $280.00
PC9-M5228-1mg (250ug x 4)
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Synonym

PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9

Source

Mouse PCSK9, His Tag (PC9-M5228) is expressed from human 293 cells (HEK293). It contains AA Gln 35 - Gln 694 (Accession # AAH38085.1).

Predicted N-terminus: Gln 35

Molecular Characterization

PCSK9(Gln 35 - Gln 694)AAH38085.1
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 72 kDa. The protein migrates as 20 kDa and 64 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation and proteolytic digestion.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>97% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Mouse PCSK9, His Tag (Cat. No. PC9-M5228) SDS-PAGE gel

Mouse PCSK9, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 97%.

Bioactivity

Mouse PCSK9, His Tag (Cat. No. PC9-M5228) ELISA bioactivity

Immobilized Human LDL R, Strep Tag (Cat. No. LDR-H5281) at 10 μg/mL (100 μl/well) can bind Mouse PCSK9, His Tag (Cat. No. PC9-M5228 ) with a linear range of 2-30 ng/mL (QC tested).

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Background

Proprotein convertase subtilisin/kexin type 9 (PCSK9) is also known as NARC1 (neural apoptosis regulated convertase), is a newly identified subtilase belonging to the peptidase S8 subfamily. Mouse PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted active enzyme with a broad alkaline pH optimum. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.