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Human CD19 (20-291) Protein  pdf  pdf  pdf


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Synonym

CD19,B4,CVID3,MGC12802

Source

Human CD19 (20-291), His Tag (CD9-H5226) is expressed from human 293 cells (HEK293). It contains AA Pro 20 - Lys 291 (Accession # AAH06338).

Predicted N-terminus: Pro 20

Molecular Characterization

CD19(Pro 20 - Lys 291)AAH06338
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 31.6 kDa. The protein migrates as 40-65 kDa under reducing (R) condition (SDS-PAGE) due to different extent glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human CD19 (20-291), His Tag (Catalog # CD9-H5226) SDS-PAGE gel

Human CD19 (20-291), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Human CD19 (20-291), His Tag (Catalog # CD9-H5226) ELISA bioactivity

Immobilized Anti-CD19 MAb, Mouse IgG2a at 2 μg/mL (100 μL/well) can bind Human CD19, His Tag (Catalog # CD9-H5226) with a linear range of 39-313 ng/mL.

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Background

B-lymphocyte antigen CD19 is also known as CD19 (Cluster of Differentiation 19), is a single-pass type I  membrane protein which contains two Ig-like C2-type (immunoglobulin-like) domains. CD19 is expressed on follicular dendritic cells and B cells. In fact, it is present on B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. It primarily acts as a B cell co-receptor in conjunction with CD21 and CD81. Upon activation, the cytoplasmic tail of CD19 becomes phosphorylated, which leads to binding by Src-family kinases and recruitment of PI-3 kinase. As on T cells, several surface molecules form the antigen receptor and form a complex on B lymphocytes. The (almost) B cell-specific CD19 phosphoglycoprotein is one of these molecules. The others are CD21 and CD81. These surface immunoglobulin (sIg)-associated molecules facilitate signal transduction. On living B cells, anti-immunoglobulin antibody mimicking exogenous antigen causes CD19 to bind to sIg and internalize with it. The reverse process has not been demonstrated, suggesting that formation of this receptor complex is antigen-induced. This molecular association has been confirmed by chemical studies. Mutations in CD19 are associated with severe immunodeficiency syndromes characterized by diminished antibody production. CD19 has been shown to interact with: CD81, CD82, Complement receptor 2, and VAV2.

References

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