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Your Position: Home > Protein > IL-17F > ILF-H5244

Human IL-17F Protein, His Tag (MALS verified)

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  • Synonym
    IL-17F,Interleukin-17F,Cytokine ML-1
  • Source
    Human IL-17F, His Tag(ILF-H5244) is expressed from human 293 cells (HEK293). It contains AA Arg 31 - Gln 163 (Accession # Q96PD4-1).
    Predicted N-terminus: His
  • Molecular Characterization
    IL-17F Structure

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 16.8 kDa. The protein migrates as 22-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-17F SDS-PAGE

Human IL-17F, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
IL-17F MALS images

The purity of Human IL-17F, His Tag (Cat. No. ILF-H5244) is more than 90% and the molecular weight of this protein is around 35-45 kDa verified by SEC-MALS.

Bioactivity-ELISA
 IL-17F ELISA

Immobilized Human IL-17F, His Tag (Cat. No. ILF-H5244) at 5 μg/mL (100 μL/well) can bind Human IL-17 RA Protein, Fc Tag (Cat. No. ILA-H525f) with a linear range of 2-39 ng/mL (QC tested).

 IL-17F ELISA

Immobilized Human IL-17F, His Tag (Cat. No. ILF-H5244) at 2 μg/mL (100 μL/well) can bind Human IL-17 RC, Fc Tag (Cat. No. ILC-H5259) with a linear range of 0.016-0.25 μg/mL (Routinely tested).

Bioactivity-BLI
 IL-17F BLI

Loaded Human IL-17 RA, Fc Tag on Protein A Biosensor, can bind Human IL-17F, His Tag (Cat. No. ILF-H5244) with an affinity constant of 219 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

 IL-17F BLI

Loaded Human IL17RA & IL17RC Protein, Fc Tag&Fc Tag (Cat. No. ILC-H5257) on Protein A Biosensor, can bind Human IL-17F, His Tag (Cat. No. ILF-H5244) with an affinity constant of 1.04 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

 IL-17F BLI

Loaded Human IL-17F, His Tag (Cat. No. ILF-H5244) on NTA Biosensor, can bind Human IL-17 RC, Fc Tag (Cat. No. ILC-H5259) with an affinity constant of 100 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Interleukin-17F (IL17F) is also known as Interleukin-24 (IL24), Cytokine ML-1, is a secreted disulfide-linked homodimer which belongs to the IL-17 family. There are at least six members of the IL-17 family in humans and in mice: IL-17A, IL-17B, IL-17C, IL-17D, IL-17E (also called IL-25) and IL-17F (also called IL-24). IL-17 represents a family of structurally related cytokines that share a highly conserved C-terminal region but differ from one another in their N-terminal regions and in their distinct biological roles. IL-17F / IL-24 is expressed in activated, but not resting, CD4+ T-cells and activated monocytes. IL17F / Interleukin-24 stimulates the production of other cytokines such as IL-6, IL-8 and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. IL17F / IL-24 stimulates PBMC and T-cell proliferation and Inhibits angiogenesis. Defects in IL17F are the cause of familial candidiasis type 6 (CANDF6).
  • Clinical and Translational Updates

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