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ActiveMax® Recombinant Human IL-4  pdf  pdf  pdf


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IL4-H4218-20ug
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IL4-H4218-50ug
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Synonym

IL4,BCGF1,BSF1

Source

ActiveMax® Human IL-4 (IL4-H4218) is expressed from human 293 cells (HEK293). It contains AA His 25 - Ser 153 (Accession # AAH67514).

Predicted N-terminus: His 25

Molecular Characterization

IL-4(His 25 - Ser 153)AAH67514

This protein carries no "tag".

The protein has a calculated MW of 14.9 kDa. The protein migrates as 15 and 19 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 0.1 EU per μg by the LAL method.

 

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS and NaCl. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

ActiveMax® Human IL-4 (Cat. No. IL4-H4218) SDS-PAGE gel

ActiveMax® Human IL-4 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity

ActiveMax® Human IL-4 (Cat. No. IL4-H4218) ELISA bioactivity

Immobilized Human IL-4, Tag Free (Cat. No. IL4-H4218) at 5μg/mL (100 μL/well) can bind Human IL-4 R alpha, His Tag (Cat. No. ILR-H5221) with a linear range of 0.01-0.1 μg/mL (QC tested).

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Background

Interleukin-4, is a cytokine that induces differentiation of naive helper T cells (Th0 cells to Th2 cells). In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, And this cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. Recently, researcher found that the cytokine IL-4 plays a key role in development of innate CD8+ T cells in the thymus of several gene-deficient mouse strains, including Itk, KLF2, CBP and Id3, without previous exposure to antigen.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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